Time point-dependent concordance of flow cytometry and real-time quantitative polymerase chain reaction for minimal residual disease detection in childhood acute lymphoblastic leukemia.

Haematologica
Giuseppe GaipaGiuseppe Basso

Abstract

Flow cytometric analysis of leukemia-associated immunophenotypes and polymerase chain reaction-based amplification of antigen-receptor genes rearrangements are reliable methods for monitoring minimal residual disease. The aim of this study was to compare the performances of these two methodologies in the detection of minimal residual disease in childhood acute lymphoblastic leukemia. Polymerase chain reaction and flow cytometry were simultaneously applied for prospective minimal residual disease measurements at days 15, 33 and 78 of induction therapy on 3565 samples from 1547 children with acute lymphoblastic leukemia enrolled into the AIEOP-BFM ALL 2000 trial. The overall concordance was 80%, but different results were observed according to the time point. Most discordances were found at day 33 (concordance rate 70%) in samples that had significantly lower minimal residual disease. However, the discordance was not due to different starting materials (total versus mononucleated cells), but rather to cell input number. At day 33, cases with minimal residual disease below or above the 0.01% cut-off by both methods showed a very good outcome (5-year event-free survival, 91.6%) or a poor one (5-year event-free survival, 50.9%), res...Continue Reading

References

Mar 6, 2002·Blood·Michael N DworzakUNKNOWN Austrian Berlin-Frankfurt-Münster Study Group
Mar 22, 2002·The Lancet Oncology·T SzczepańskiJ J van Dongen
May 4, 2002·Best Practice & Research. Clinical Haematology·Dario Campana, Elaine Coustan-Smith
Jun 6, 2003·British Journal of Haematology·Dario Campana
Feb 9, 2007·Leukemia·V H J van der VeldenUNKNOWN European Study Group on MRD detection in ALL (ESG-MRD-ALL)
Feb 14, 2008·Cytometry. Part B, Clinical Cytometry·Giuseppe GaipaUNKNOWN I-BFM-ALL-FCM-MRD-Study Group
Oct 7, 2009·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Giuseppe BassoGiuseppe Gaipa

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Citations

Mar 1, 2014·Archives of Pathology & Laboratory Medicine·Min ShiHongbo Yu
Dec 17, 2015·Revista brasileira de hematologia e hemoterapia·Maura Rosane Valério IkomaUNKNOWN Minimal Residual Disease Working Group of the Brazilian Society of Bone Marrow Transplantation (SBTMO)
Jun 13, 2013·Cytometry. Part B, Clinical Cytometry·Giuseppe GaipaDario Campana
Mar 26, 2014·Journal of Traditional Chinese Medicine = Chung I Tsa Chih Ying Wen Pan·Huang ZhuXianqun Fan
Oct 4, 2012·Blood·Monika BrüggemannMichael Kneba
Jul 22, 2016·Journal of Pediatric Hematology/oncology·Min XiaHong Li
Nov 30, 2010·Best Practice & Research. Clinical Haematology·Shaji KumarWilliam Morice
Oct 8, 2014·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Maddalena PaganinGiuseppe Basso
Mar 4, 2018·International Journal of Laboratory Hematology·E TsitsikovO K Weinberg
Jul 27, 2018·Expert Review of Molecular Diagnostics·Giuseppe GaipaA Biondi
Jul 10, 2019·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Michael ReiterUNKNOWN International Berlin-Frankfurt-Münster (iBFM)-FLOW-network and the AutoFLOW consortium
Feb 17, 2017·Current Oncology Reports·Nicholas J Short, Elias Jabbour
Apr 30, 2017·Molecular Diagnosis & Therapy·Michaela KotrovaMonika Brüggemann
Mar 28, 2020·Therapeutic Advances in Hematology·Iman Abou DalleNicholas J Short
Nov 18, 2018·Current Hematologic Malignancy Reports·Franklin Fuda, Weina Chen
May 6, 2017·Cytometry. Part B, Clinical Cytometry·Michael KeeneyMichael J Borowitz

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