Time-related thyroid stimulation by thyrotropin and thyroid-stimulating antibodies, as measured by the cytochemical section bioassay

The Journal of Clinical Endocrinology and Metabolism
P A EaleyR P Ekins

Abstract

The time course of response of thyroid sections in the cytochemical bioassay to either TSH or thyroid-stimulating antibodies is bell shaped. The maximal staining for lysosomal naphthylamidase activity achieved was found to be the same regardless of the dose of stimulator applied; however, the rate at which the maximum was attained was dose dependent. Sections exposed to 10(-1) mU/liter TSH showed a maximal response at 120 sec, and those exposed to 10(-3) mU/liter TSH showed a maximal response at 210 sec. A similar dose-time effect was seen with immunoglobulin G from a thyrotoxic patient. Thus, by selecting a specific exposure time, a dose-response curve to the stimulator was obtained. A dose-response curve to a range of concentrations from 10(-4)-10(-1) mU/liter TSH was obtained by exposing sections to the hormone for 90 sec. TSH (10(-3) mU/liter) produced a response significantly different (P less than 0.0025) from the control. However, 10(-5) mU/liter TSH produced a response significantly different (P less than 0.0025) from the control after an exposure time of 180 sec, and the range of the dose-response curve at this exposure time was 10(-6)-10(-3) mU/liter TSH. Each point on these two dose-response curves was determined in ...Continue Reading

Citations

Jan 1, 1982·Springer Seminars in Immunopathology·S W ManleyD D Adams
Nov 1, 1984·American Heart Journal·A SanmartíJ Soler-Soler
Jun 1, 1986·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·K SakaguchiT Fujita
Mar 1, 1983·Clinics in Endocrinology and Metabolism·T F Davies
May 1, 1985·Clinics in Endocrinology and Metabolism·A A Gossage, D S Munro
Aug 1, 1987·Clinical Endocrinology·W R RobertsonN Loveridge
Oct 1, 1984·Cell Biochemistry and Function·D JolinkR D van der Gaag

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