PMID: 9545536Apr 18, 1998Paper

Time resolved absorption study of the reaction of hydroxyurea with sickle cell hemoglobin

Biochimica Et Biophysica Acta
Daniel B Kim-ShapiroSamir K Ballas

Abstract

Hydroxyurea has been mixed with hemoglobin S and the reaction was studied using electronic absorption spectroscopy as a function of time and wavelength. The rate of conversion of oxyhemoglobin S to other species was determined and the nature of the reaction products was studied. We also report the formation of methemoglobin (and other reaction products) when deoxyhemoglobin S is combined with hydroxyurea. The probable increase in the formation of methemoglobin, and other potential reaction products such as nitric oxide-hemoglobin, in patients with sickle cell anemia who are taking hydroxyurea as a therapeutic drug is discussed in terms of the pathophysiology of the disease. It is proposed that methemoglobin and possibly nitric oxide-hemoglobin formation may partially explain beneficial effects observed in these patients before their levels of fetal hemoglobin have increased.

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Citations

Aug 12, 2004·Free Radical Biology & Medicine·S Bruce King
Mar 19, 2003·Blood Reviews·Sally C Davies, Annette Gilmore
Jul 26, 2003·Biochimica Et Biophysica Acta·Virginia L LockamyDaniel B Kim-Shapiro
Feb 14, 2002·British Journal of Haematology·Mark T GladwinGriffin P Rodgers
Mar 11, 2003·American Journal of Respiratory and Critical Care Medicine·Claudia R MorrisElliott P Vichinsky
Jan 18, 2003·The Journal of Clinical Investigation·Vladan P CokicAlan N Schechter
Feb 12, 2008·Free Radical Biology & Medicine·Katherine C WoodMark T Gladwin
Mar 23, 2004·Analytical Biochemistry·Joaquim JaumotRaimundo Gargallo
Jun 8, 2001·American Journal of Hematology·Z HuangD B Kim-Shapiro
Apr 12, 2006·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Betty S Pace, Sima Zein
Feb 13, 2003·Current Opinion in Hematology·Christopher D Reiter, Mark T Gladwin
Aug 7, 2003·Journal of Pediatric Hematology/oncology·Claudia R MorrisFrans A Kuypers
Nov 13, 2007·American Journal of Physiology. Heart and Circulatory Physiology·Johnson HaynesTroy Stevens
Apr 11, 2002·Chemical Reviews·Peng George WangAdam J Janczuk
Aug 6, 1999·Biochimica Et Biophysica Acta·D B Kim-ShapiroS K Ballas
Mar 14, 2007·Biophysical Chemistry·Kelly M KneeIshita Mukerji
May 28, 2008·Free Radical Biology & Medicine·Sonia DonzelliDavid A Wink

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