Time-resolved biofilm deformation measurements using optical coherence tomography

Biotechnology and Bioengineering
Florian BlauertMichael Wagner

Abstract

The interaction of shear stress with the biofilm leads to a dynamic deformation, which is related to the structural and material characteristics of biofilms. We show how optical coherence tomography can be used as an imaging technique to investigate the time-resolved deformation on the biofilm mesoscale as well as to estimate mechanical properties of the biofilm. For the first time time-resolved deformation from cross-sectional views of the inner biofilm structure could be shown. Changes in the biofilm structure and rheological properties were calculated from cross sections in real-time and time-lapsed measurements. Heterotrophic biofilms were grown in a flow cell set-up at low shear stress of τw  = 0.01 Pa. By applying higher shear stress elastic and viscoelastic behavior of biofilms were quantified. Deformation led to a change in biofilm conformation and allowed to estimate rheological properties. Assuming an ideal wall shear stress calculation, the shear modulus G = 29.7 ± 1.7 Pa and the Young's modulus E = 36.0 ± 2.6 Pa were estimated.

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Citations

Sep 16, 2015·Biotechnology and Bioengineering·Michele ZanoniEoin Casey
Mar 8, 2017·Biotechnology and Bioengineering·Michael Wagner, Harald Horn
Aug 12, 2017·Environmental Microbiology·Stefania FabbriPaul Stoodley
May 20, 2018·Biotechnology and Bioengineering·Alex F RosenthalGeorge F Wells
Mar 16, 2016·Journal of Bacteriology·Karen L VisickJean-Marc Ghigo
Oct 5, 2016·Biomedical Optics Express·Nicolás WeissTon G van Leeuwen
Mar 19, 2016·Journal of Biophotonics·Curtis LarimerRaymond Shane Addleman
Apr 6, 2018·Clinical Microbiology Reviews·Maria MaganaGeorge P Tegos
Aug 23, 2018·NPJ Biofilms and Microbiomes·Héloïse BoudarelMichel Grédiac
Mar 3, 2017·Journal of the Royal Society, Interface·Daniel WangpraseurtMichael Kühl
May 13, 2019·Applied Microbiology and Biotechnology·Miriam EdelJohannes Gescher
Aug 7, 2019·International Journal of Molecular Sciences·Cristina Cattò, Francesca Cappitelli
Jul 14, 2020·Biotechnology and Bioengineering·Mengfei LiRobert Nerenberg
Aug 8, 2020·Nanoscale·Christian Titus Kreis, Ruby May A Sullan
Nov 21, 2020·The Science of the Total Environment·Kévin LequetteNathalie Wéry

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