Time-resolved dimerization of a PAS-LOV protein measured with photocoupled small angle X-ray scattering.

Journal of the American Chemical Society
Jessica LambLois Pollack

Abstract

Time-resolved small-angle X-ray scattering (SAXS) has been used to probe photoexcitation of the blue-light signal transduction protein Vivid (VVD). Laser excitation of sample in a continuous flow cell enables time-resolved measurement of the initial response of VVD to illumination. Good signal-to-noise is achieved without relying on multiple exposures of the same sample or limiting exposure times to prevent radiation damage. The SAXS data demonstrate that VVD dimerizes within tens of milliseconds of light-state activation. Time-resolved SAXS in a flow cell format is a general method for connecting chemical changes in photoreceptors to conformationally driven output signals.

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Citations

Sep 3, 2010·Proceedings of the National Academy of Sciences of the United States of America·Suzanne M HuntChristian Heintzen
Aug 25, 2010·Proceedings of the National Academy of Sciences of the United States of America·Chen-Hui ChenJennifer J Loros
Aug 27, 2011·Science Signaling·Anand T VaidyaBrian R Crane
Jun 7, 2014·Annual Review of Biochemistry·Brian R Crane, Michael W Young
Nov 6, 2013·Journal of Molecular Biology·Chung-Tien LeeMatthias P Mayer
Aug 29, 2009·Journal of Molecular Biology·Jessica S LambLois Pollack
Jul 3, 2015·Frontiers in Molecular Biosciences·Thea Ziegler, Andreas Möglich
May 20, 2015·Frontiers in Molecular Biosciences·Ashutosh PudasainiBrian D Zoltowski
Jun 19, 2010·Journal of Structural Biology·Haydyn D T Mertens, Dmitri I Svergun
Jul 17, 2018·PloS One·Katrin RöllenAndreas Maximilian Stadler
Feb 19, 2010·Acta Crystallographica. Section A, Foundations of Crystallography·Sebastian WestenhoffRichard Neutze
Mar 10, 2011·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Giuliano Zanchetta, Roberto Cerbino
May 23, 2019·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Vera V NazarenkoIvan Gushchin
Jun 9, 2017·Annual Review of Chemical and Biomolecular Engineering·Nicole A RepinaRavi S Kane
Apr 18, 2012·Journal of Applied Crystallography·S S NielsenR E Gillilan
Feb 18, 2011·Biopolymers·Lois Pollack
Mar 21, 2017·Methods : a Companion to Methods in Enzymology·Jamie L BingamanPhilip C Bevilacqua
Jul 10, 2018·Chemical Reviews·Aba LosiAndreas Möglich

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