Time-resolved dual transcriptomics reveal early induced Nicotiana benthamiana root genes and conserved infection-promoting Phytophthora palmivora effectors

BMC Biology
Edouard EvangelistiSebastian Schornack

Abstract

Plant-pathogenic oomycetes are responsible for economically important losses in crops worldwide. Phytophthora palmivora, a tropical relative of the potato late blight pathogen, causes rotting diseases in many tropical crops including papaya, cocoa, oil palm, black pepper, rubber, coconut, durian, mango, cassava and citrus. Transcriptomics have helped to identify repertoires of host-translocated microbial effector proteins which counteract defenses and reprogram the host in support of infection. As such, these studies have helped in understanding how pathogens cause diseases. Despite the importance of P. palmivora diseases, genetic resources to allow for disease resistance breeding and identification of microbial effectors are scarce. We employed the model plant Nicotiana benthamiana to study the P. palmivora root infections at the cellular and molecular levels. Time-resolved dual transcriptomics revealed different pathogen and host transcriptome dynamics. De novo assembly of P. palmivora transcriptome and semi-automated prediction and annotation of the secretome enabled robust identification of conserved infection-promoting effectors. We show that one of them, REX3, suppresses plant secretion processes. In a survey for early tr...Continue Reading

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Citations

Apr 5, 2018·Proceedings of the National Academy of Sciences of the United States of America·Philip CarellaSebastian Schornack
Oct 27, 2017·Molecular Plant-microbe Interactions : MPMI·Rebecca GumtowMiaoying Tian
Sep 13, 2020·Journal of Fungi·Francine Perrine-Walker
Dec 17, 2020·International Journal of Molecular Sciences·Miroslav BerkaMartin Černý
Dec 10, 2020·International Journal of Molecular Sciences·Yee-Shan KuHon-Ming Lam
Jun 3, 2021·Microorganisms·Karen Cristine Goncalves Dos SantosHugo Germain
Aug 14, 2021·Science·Federico SabbadinSimon J McQueen-Mason

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Datasets Mentioned

BETA
KX130348
KX130352
X06930.1
SRP096022
UP000018817

Methods Mentioned

BETA
GTPase
transgenic
PCA
ubiquitination
PCR
amplicon sequencing
fluorescence microscopy
transgenics
electrophoresis

Software Mentioned

InterProsScan
bedtools
Vennerable
bowtie2
R ’ cluster
BUSCO
CEGMA
SignalP
HMM3
topGO

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