Time-Resolved Fluorescence Detection of Superoxide Anions Based on an Enzyme-Integrated Lanthanide Coordination Polymer Composite

ACS Applied Materials & Interfaces
Yuhao WengHongliang Tan

Abstract

In this work, we proposed a new strategy of fabricating time-resolved fluorescent nanoprobes by using an enzyme-integrated lanthanide coordination polymer (CP) composite for the detection of superoxide anions (O2•-). This CP composite was constructed with terbium ions (Tb3+) as a metal node, adenosine triphosphate (ATP) as a bridge ligand, and carboxyphenylboronic acid (CPBA) as a sensitizer in which superoxide dismutase (SOD) was encapsulated by a self-adaptive inclusion process. The as-prepared SOD@ATP/Tb-CPBA displays both catalytic and fluorescence properties. Benefiting from the shielding effect of ATP/Tb CP, greatly enhanced catalytic activity and stability against harsh environments can be obtained in the loaded SOD. Meanwhile, the loaded SOD can remove the water molecules on the coordination sphere of Tb3+, leading to a significant increase in the fluorescence intensity and lifetime of SOD@ATP/Tb-CPBA. However, upon the addition of O2•-, the fluorescence of SOD@ATP/Tb-CPBA was quenched significantly. This is because SOD can convert O2•- into H2O2 to induce the deboronation of CPBA, resulting in an intramolecular charge transfer process. On this basis, by taking advantage of Tb3+ in long lifetime emission, a time-resolve...Continue Reading

References

Mar 4, 2000·Clinica Chimica Acta; International Journal of Clinical Chemistry·A V Peskin, C C Winterbourn
Aug 18, 2001·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·H SauerJ Hescheler
May 3, 2005·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Joe M McCord, Marvin A Edeas
Mar 14, 2008·Journal of the American Chemical Society·Duangkhae SrikunChristopher J Chang
Jan 27, 2009·Journal of the American Chemical Society·Ryuhei NishiyabuNobuo Kimizuka
Feb 2, 2010·Analytical Biochemistry·Luciane Dos Santos TeixeiraLilia Coronato Courrol
Jan 14, 2012·Chemistry : a European Journal·Yaling Liu, Zhiyong Tang
Mar 28, 2013·Chemical Society Reviews·Roger A Sheldon, Sander van Pelt
Apr 27, 2013·Nature Reviews. Immunology·Carl Nathan, Amy Cunningham-Bussel
Oct 22, 2013·Biotechnology and Bioengineering·Feng JiaSurya Mallapragada
Nov 30, 2013·Nature Reviews. Drug Discovery·Chiara GorriniTak W Mak
Apr 4, 2014·ACS Applied Materials & Interfaces·Feng-Peng ChangChung-Yuan Mou
Feb 16, 2016·Chemical Reviews·Maan HayyanInas M AlNashef
Dec 27, 2016·Free Radical Biology & Medicine·Alexander V Peskin, Christine C Winterbourn
Mar 28, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Baoxia LiuYang Chen

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Citations

Jun 20, 2021·Advanced Science·Jiating XuYujie Fu

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