PMID: 9433472Jan 20, 1998Paper

Time-resolved fluorescence imaging in islet cell autoantibody quantitation

Journal of Immunological Methods
M RulliO Simell

Abstract

The prodromal period of insulin-dependent diabetes mellitus (IDDM) is characterized by circulating islet cell autoantibodies (ICA) and other beta cell specific autoantibodies. Despite biochemical characterization of the major beta cell autoantigens insulin, glutamic acid decarboxylase and protein tyrosine phosphatase and development of the respective antibody assays, ICA has remained the standard in IDDM prediction. Conventional ICA quantitation using classic fluorochromes is prone to errors since fluorescence intensity is estimated subjectively using the human eye, which is also unable to differentiate specific signals from non-specific signals and autofluorescence. Using Eu(3+)-chelate labelled anti-human polyclonal IgG (decay time 1000 microseconds) as the secondary antibody in time-resolved fluorescence imaging (TRFI), the chelate and autofluorescence signals (typical decay time < 100 ns) are fully separated. The image is recorded using an optically gated cooled digital CCD camera. The specificity of the ICA signal is further improved by interactive analysis of the image. Signal detection is objective, the signal-to-background ratio improves, and ICA quantitation is possible using undiluted serum. Of 57 consecutive new-onse...Continue Reading

References

Mar 1, 1985·Diabetes·S SrikantaG S Eisenbarth
Mar 1, 1984·Analytical Biochemistry·I HemmiläT Lövgren

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Citations

May 11, 2011·Analytical and Bioanalytical Chemistry·A K Hagan, T Zuchner
Nov 26, 2015·Chemical Communications : Chem Comm·Nima SayyadiAndrew Try
Jul 4, 2008·Annals of the New York Academy of Sciences·Russell E Connally, James A Piper
Nov 30, 2006·Annals of the New York Academy of Sciences·Pauli VuorinenOlli Simell
Jan 19, 2002·Annals of the New York Academy of Sciences·D HunkelerN Angelova
Feb 16, 2010·Chemical Reviews·Jean-Claude G Bünzli

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