Time-resolved fluorescence studies on site-directed mutants of human serum albumin

FEBS Letters
M K HelmsD M Jameson

Abstract

Human serum albumin (HSA) contains a single tryptophan residue at position 214. The emission properties of tryptophan 214 from recombinant albumins, namely, normal HSA, FDH-HSA and a methionine 218 HSA were examined. In all cases, the excited state lifetimes were best described by a two component model consisting mainly of a Lorentzian distribution. The centers of these distributions were 5.60 ns for HSA, 4.23 ns for FDH-HSA, and 6.08 ns for Met-218 HSA. The global rotational correlation times of the three HSAs were near 41 ns while the amplitude and rate of the local motion varied. These changes in the lifetimes and mobilities suggest perturbation in the local protein environment near tryptophan 214 as a consequence of the amino acid substitutions.

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Citations

Dec 31, 2005·Journal of Fluorescence·Denisio M Togashi, Alan G Ryder
Oct 4, 2008·Journal of Combinatorial Chemistry·Matthew D ShortridgeRobert Powers
Feb 11, 2014·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Kulwinder SagooGraham Hungerford
Feb 25, 2014·Chemico-biological Interactions·Bigyan R JaliJubaraj B Baruah
Jan 1, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Nirnay SamantaMartina Havenith
Nov 17, 2010·Analytical Biochemistry·Nicholas G JamesDavid M Jameson
Sep 17, 2013·Protein Science : a Publication of the Protein Society·Rajeev Yadav, Pratik Sen
May 7, 2013·Biochimica Et Biophysica Acta·Jörg Reichenwallner, Dariush Hinderberger
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Oct 26, 2016·Journal of Photochemistry and Photobiology. B, Biology·Selva Sharma ArumugamIlanchelian Malaichamy
Oct 30, 2008·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Justin A Ross, David M Jameson
Apr 4, 2007·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Sandra MontiSalvatore Sortino
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Jun 7, 2011·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Xinhu WuJing Jin

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