Time-resolved FT-IR microspectroscopy of protein aggregation induced by heat-shock in live cells

Analytical Chemistry
Elisa MitriLisa Vaccari

Abstract

Maintaining the correct folding of cellular proteins is essential for preserving cellular homeostasis. Protein dishomeostasis, aberrant protein folding, and protein aggregation are indeed involved in several diseases including cancer, aging-associated, and neurodegenerative disorders. Accumulation of protein aggregates can also be induced from a variety of stressful conditions, such as temperature increase or oxidative stress. In this work, we monitored by Fourier transform-infrared (FT-IR) microspectroscopy the response of live breast cancer MCF-7 and mammary breast adenocarcinoma MDA-MB 231 cell lines to severe heat-shock (HS), caused by the rise of the cellular medium temperature from 37 ± 0.5 °C to 42 ± 0.5 °C. Through the study of the time-evolution of the second derivatives of the spectra and by the 2D correlation analysis of FT-IR absorbance data, we were able to identify a common sudden heat-shock response (HSR) among the two cell lines. The hyperfluidization of mammalian cell membranes, the transient increment of the signal lipids, as well as the alteration of proteome profile were all monitored within the first 40 min of stress application, while the persistent intracellular accumulation of extended β-folded protein a...Continue Reading

References

Jan 1, 1986·Annual Review of Biochemistry·S Lindquist
Apr 14, 1980·Biochemical and Biophysical Research Communications·L McAlister, D B Finkelstein
Nov 23, 2000·Nature·T Finkel, N J Holbrook
Feb 28, 2001·Progress in Biophysics and Molecular Biology·A Barth
Jul 6, 2002·Critical Reviews in Oncology/hematology·Bert HildebrandtHanno Riess
Oct 9, 2002·Proceedings of the National Academy of Sciences of the United States of America·Nelly M TsvetkovaLászló Vigh
Jul 25, 2003·Biophysical Chemistry·Martina BanyayAstrid Gräslund
Nov 3, 2004·Biochimica Et Biophysica Acta·Dmitry A Los, Norio Murata
Feb 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·J Bandekar, S Krimm
Aug 19, 2007·Biochimica Et Biophysica Acta·Andreas Barth
Feb 16, 2008·Science·William E BalchJeffery W Kelly
Mar 5, 2009·Journal of the American Chemical Society·Lauren P DefloresAndrei Tokmakoff
Sep 16, 2010·Analytical Chemistry·Hoi-Ying N HolmanMichael C Martin
Oct 12, 2010·Analytical and Bioanalytical Chemistry·Elisabetta GiorginiOliana Carnevali
Oct 23, 2010·Molecular Cell·Klaus RichterJohannes Buchner
Dec 3, 2010·Cold Spring Harbor Perspectives in Biology·R Martin VabulasF Ulrich Hartl
Jan 30, 2013·Biochimica Et Biophysica Acta·Lisa M MillerRandy J Smith
May 11, 2013·The Analyst·Diana E BedollaLisa Vaccari
May 21, 2013·FEBS Letters·Gábor BaloghLászló Vígh
May 21, 2013·FEBS Letters·Smriti PriyaPierre Goloubinoff
Jun 6, 2014·Journal of the Royal Society, Interface·Donna R WhelanBayden R Wood

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Citations

Oct 22, 2015·Chemical Society Reviews·K L Andrew Chan, Sergei G Kazarian
Mar 14, 2019·The Analyst·Ali AltharawiK L Andrew Chan
Jul 23, 2018·Analytical and Bioanalytical Chemistry·K L Andrew ChanGianfelice Cinque

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