Time-resolved luminescence detection of peroxynitrite using a reactivity-based lanthanide probe.

Chemical Science
Colum BreenStephen J Butler

Abstract

Peroxynitrite (ONOO-) is a powerful and short-lived oxidant formed in vivo, which can react with most biomolecules directly. To fully understand the roles of ONOO- in cell biology, improved methods for the selective detection and real-time analysis of ONOO- are needed. We present a water-soluble, luminescent europium(iii) probe for the rapid and sensitive detection of peroxynitrite in human serum, living cells and biological matrices. We have utilised the long luminescence lifetime of the probe to measure ONOO- in a time-resolved manner, effectively avoiding the influence of autofluorescence in biological samples. To demonstrate the utility of the Eu(iii) probe, we monitored the production of ONOO- in different cell lines, following treatment with a cold atmospheric plasma device commonly used in the clinic for skin wound treatment.

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Citations

Jun 25, 2021·Chemical Science·Samantha E Bodman, Stephen J Butler
Apr 29, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Elena Del Giorgio, Thomas Just Sørensen
Jun 3, 2021·Chemical Society Reviews·David ParkerKa-Leung Wong
Jul 15, 2021·Chemical Science·Jake L GreenfieldMatthew J Fuchter

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Methods Mentioned

BETA
X-ray
confocal microscopy
fluorescence microscopy

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