Time-resolved optical absorption microspectroscopy of magnetic field sensitive flavin photochemistry

The Review of Scientific Instruments
Lewis M AntillJonathan R Woodward

Abstract

The photochemical reactions of blue-light receptor proteins have received much attention due to their very important biological functions. In addition, there is also growing evidence that the one particular class of such proteins, the cryptochromes, may be associated with not only a biological photo-response but also a magneto-response, which may be responsible for the mechanism by which many animals can respond to the weak geomagnetic field. Therefore, there is an important scientific question over whether it is possible to directly observe such photochemical processes, and indeed the effects of weak magnetic fields thereon, taking place both in purified protein samples in vitro and in actual biochemical cells and tissues. For the former samples, the key lies in being able to make sensitive spectroscopic measurements on very small volumes of samples at potentially low protein concentrations, while the latter requires, in addition, spatially resolved measurements on length scales smaller than typical cellular components, i.e., sub-micron resolution. In this work, we discuss a two- and three-color confocal pump-probe microscopic approach to this question which satisfies these requirements and is thus useful for experimental meas...Continue Reading

References

Feb 2, 2000·Biophysical Journal·T RitzK Schulten
May 2, 2008·Nature·Kiminori MaedaP J Hore
Jul 22, 2008·Nature·Robert J GegearSteven M Reppert
Mar 17, 2012·Proceedings of the National Academy of Sciences of the United States of America·Kiminori MaedaP J Hore
Sep 4, 2012·International Journal of Molecular Sciences·Alfons Penzkofer
Jun 4, 2015·Angewandte Chemie·Joshua P BeardmoreJonathan R Woodward
Oct 17, 2015·Annual Review of Entomology·Steven M ReppertChristine Merlin
Mar 24, 2016·Nature Chemistry·Daniel R KattnigP J Hore
May 25, 2016·Annual Review of Biophysics·P J Hore, Henrik Mouritsen
Feb 9, 2017·Scientific Reports·Dean M W SheppardStuart R Mackenzie

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Citations

Dec 24, 2020·Physical Chemistry Chemical Physics : PCCP·Marcin KonowalczykJonathan G Storey
Jan 6, 2021·Proceedings of the National Academy of Sciences of the United States of America·Noboru Ikeya, Jonathan R Woodward
May 5, 2018·The Journal of Physical Chemistry Letters·Lewis M Antill, Jonathan R Woodward
Nov 27, 2019·The Journal of Physical Chemistry. B·Manabu SakuraiTakashi Tachikawa

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