Time-resolved single-step protease activity quantification using nanoplasmonic resonator sensors.

ACS Nano
Cheng SunXiang Zhang

Abstract

Protease activity measurement has broad application in drug screening, diagnosis and disease staging, and molecular profiling. However, conventional immunopeptidemetric assays (IMPA) exhibit low fluorescence signal-to-noise ratios, preventing reliable measurements at lower concentrations in the clinically important picomolar to nanomolar range. Here, we demonstrated a highly sensitive measurement of protease activity using a nanoplasmonic resonator (NPR). NPRs enhance Raman signals by 6.1 x 10(10) times in a highly reproducible manner, enabling fast detection of proteolytically active prostate-specific antigen (paPSA) activities in real-time, at a sensitivity level of 6 pM (0.2 ng/mL) with a dynamic range of 3 orders of magnitude. Experiments on extracellular fluid (ECF) from the paPSA-positive cells demonstrate specific detection in a complex biofluid background. This method offers a fast, sensitive, accurate, and one-step approach to detect the proteases' activities in very small sample volumes.

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Citations

Mar 10, 2011·ACS Nano·Xiaodong YangXiang Zhang
Mar 29, 2012·Applied Physics Letters·Michael Ian LapsleyTony Jun Huang
Jan 4, 2013·Journal of Applied Physics·Mengqian LuTony Jun Huang
Apr 17, 2012·Applied Optics·Arindam Dasgupta, G V Pavan Kumar
Jan 4, 2012·Proteomics. Clinical Applications·Peter Findeisen, Michael Neumaier
Mar 6, 2015·Chemical Society Reviews·Li Wu, Xiaogang Qu
Nov 27, 2010·The Analyst·Dongmao ZhangAndrzej Sygula
Mar 21, 2017·ACS Nano·Hao WangOlivier J F Martin
May 11, 2017·The Analyst·Irvine Lian Hao Ong, Kun-Lin Yang
Sep 19, 2017·Faraday Discussions·Pieter C WuytensRoel Baets
Oct 23, 2012·The Analyst·Ghadeer A R Y SuaifanMohammed Zourob
Oct 21, 2018·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Fei MaChun-Yang Zhang
Jun 2, 2021·Optics Letters·Michael DubrovskyDiedrik Vermeulen

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