Time-resolved singlet oxygen phosphorescence measurements from photosensitized experiments in single cells: effects of oxygen diffusion and oxygen concentration

Photochemistry and Photobiology
Sonja HatzPeter R Ogilby

Abstract

Time-resolved singlet oxygen, O(2)(a(1)Delta(g)), phosphorescence experiments have been performed in single cells upon pulsed laser irradiation of a photosensitizer incorporated into the cell. Data recorded as a function of the partial pressure of ambient oxygen to which the cell is exposed reflect apparent values for the intracellular oxygen diffusion coefficient and intracellular oxygen concentration that are smaller than those found in neat H(2)O. This conclusion is supported by O(2)(a(1)Delta(g)) phosphorescence data and sensitizer triplet state absorption data recorded in control experiments on sucrose solutions with different viscosities. We recently demonstrated that the intracellular lifetime of O(2)(a(1)Delta(g)) is comparatively long ( approximately 3 micros) and does not differ significantly from that in neat H(2)O ( approximately 3.5 micros). Despite this long lifetime, however, our estimate of an apparent intracellular oxygen diffusion coefficient in the range approximately 2-4 x 10(-6) cm(2) s(-1) means that the spatial domain of intracellular O(2)(a(1)Delta(g)) activity will likely have a spherical radius of approximately 100 nm. This latter point helps reconcile seeming inconsistencies between our direct O(2)(a(...Continue Reading

References

Apr 1, 1992·Photochemistry and Photobiology·A Baker, J R Kanofsky
Apr 1, 1985·Magnetic Resonance in Medicine : Official Journal of the Society of Magnetic Resonance in Medicine·P D Morse, H M Swartz
Jan 1, 1993·The Journal of Cell Biology·H P KaoA S Verkman
Oct 21, 1995·Journal of Theoretical Biology·A Dutta, A S Popel
Nov 1, 1996·Journal of Applied Physiology·M Sinaasappel, C Ince
Aug 15, 2002·Journal of Biomedical Optics·Jin JiZeev Rosenzweig
May 15, 2003·Chemical Reviews·Claude Schweitzer, Reinhard Schmidt
May 13, 2004·Photochemistry and Photobiology·Ingo ZebgerPeter R Ogilby
Jan 6, 2005·Journal of the American Chemical Society·Peter K FrederiksenPeter R Ogilby
Oct 20, 2005·Journal of the American Chemical Society·John W SnyderPeter R Ogilby
May 19, 2006·Photochemistry and Photobiology·Esben SkovsenPeter R Ogilby
Jun 3, 2006·Photochemistry and Photobiology·Robert W Redmond, Irene E Kochevar
Jul 21, 2006·The Journal of Physical Chemistry. B·Esben SkovsenPeter R Ogilby
Sep 21, 2006·Physical Chemistry Chemical Physics : PCCP·John W SnyderPeter R Ogilby
Oct 5, 2007·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Sonja HatzPeter R Ogilby

❮ Previous
Next ❯

Citations

Mar 17, 2011·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Paola CeroniSergei A Vinogradov
Aug 18, 2009·Photochemistry and Photobiology·Aisling E O'ConnorAnnette T Byrne
Dec 15, 2010·Photochemistry and Photobiology·Jeffrey R Kanofsky
Jun 15, 2011·Photochemistry and Photobiology·Brian W PedersenPeter R Ogilby
Dec 26, 2013·Proceedings of the National Academy of Sciences of the United States of America·Jie ChenPeter M Rentzepis
Oct 23, 2015·ACS Applied Materials & Interfaces·Shouying LiChangfeng Wu
May 27, 2009·Journal of Photochemistry and Photobiology. B, Biology·Ole-Jacob NorumKristian Berg
Oct 17, 2015·Journal of the American Chemical Society·Matthias Klaper, Torsten Linker
Mar 29, 2014·Photochemistry and Photobiology·Ashwini A GhogareAlexander Greer
Jul 22, 2014·Photochemistry and Photobiology·Mark A Weston, Michael S Patterson
May 18, 2016·Journal of the American Chemical Society·Matthias KlaperTorsten Linker
Oct 25, 2016·Methods : a Companion to Methods in Enzymology·Michael WestbergPeter R Ogilby
Sep 3, 2016·ACS Applied Materials & Interfaces·Petr HenkeJiří Mosinger
Jun 22, 2016·Nanoscale·Girgis ObaidTayyaba Hasan
Jan 17, 2015·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Marek ScholzJan Hála
Sep 25, 2017·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Marek ScholzJan Hála
Apr 1, 2009·Nature Chemistry·Marina K KuimovaPeter R Ogilby
Jun 10, 2010·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Ioanna StamatiMahendra P Deonarain
Jun 24, 2010·Chemical Society Reviews·Peter R Ogilby
Oct 22, 2010·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Peter R Ogilby
Nov 18, 2010·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·David Phillips
Apr 2, 2009·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Thomas BreitenbachPeter R Ogilby
Dec 4, 2013·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Mark A Weston, Michael S Patterson
Oct 5, 2019·Chemical Communications : Chem Comm·Kanchan BasnetKathryn B Grant
Dec 18, 2019·Essays in Biochemistry·Eduardo Fuentes-Lemus, Camilo López-Alarcón
Sep 9, 2020·Organic & Biomolecular Chemistry·Shunsuke SakuraiMakiko Tanaka
Apr 25, 2018·Chemistry : a European Journal·Jean-François LongevialSébastien Richeter
Jul 8, 2009·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Christel PierlotJean-Marie Aubry
Jul 8, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Effibe O AhoulouKathryn B Grant
Aug 26, 2019·Free Radical Biology & Medicine·Eduardo Fuentes-LemusMichael J Davies
Dec 5, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Valéria VerebováJana Staničová
Dec 23, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Nickolass Bitsch SchackPeter R Ogilby
Jan 9, 2009·Journal of the American Chemical Society·Marina K KuimovaPeter R Ogilby
Nov 24, 2019·Current Opinion in Chemical Biology·Sherri A McFarlandGilles Gasser
Jun 30, 2021·Photodiagnosis and Photodynamic Therapy·Pollyanna C V ConradoErika S Kioshima
Aug 5, 2021·Russian Journal of General Chemistry·S A SyrbuN Sh Lebedeva
Sep 25, 2010·ACS Nano·Nicholas StephanopoulosMatthew B Francis

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.