PMID: 6989411Apr 1, 1980Paper

Time resolved spectroscopy of tryptophyl fluorescence of yeast 3-phosphoglycerate kinase

Biophysical Chemistry
J P PrivatR H Pain

Abstract

The tryptophyl fluorescence emission of yeast 3-phosphoglycerate kinase decreases from pH 3.9 to pH 7.2 following a normal titration curve with an apparent pK of 4.7. The fluorescence decays have been determined at both extreme pH by photocounting pulse fluorimetry and have been found to vary with the emission wavelength. A quantitative analysis of these results according to a previously described method allows to determine the emission characteristics of the two tryptophan residues present in the protein molecule. At pH 3.9, one of the tryptophan residues is responsible for only 13% of the total fluorescence emission. This first residue has a lifetime tau 1 = 0.6 ns and a maximum fluorescence wavelength lambda 1max = 332 nm. The second tryptophan residue exhibits two lifetimes tau 21 = 3.1 ns and tau 22 = 7.0 ns (lambda 2max = 338 nm). In agreement with the attribution of tau 21 and tau 22 to the same tryptophan residue, the ratio beta = C21/C22 of the normalized amplitudes is constant along the fluorescence emission spectrum. At pH 7.2, the two tryptophan residues contribute almost equally to the protein fluorescence. The decay time of tryptophan 1 is 0.4 ns. The other emission parameters are the same as those determined at p...Continue Reading

References

Dec 21, 1977·Biochemical and Biophysical Research Communications·J C BrochonC Hélène
Nov 15, 1973·Journal of Molecular Biology·G E SchulzR H Schirmer

❮ Previous
Next ❯

Citations

Apr 16, 2003·Journal of Photochemistry and Photobiology. B, Biology·Roopa Kenoth, Musti J Swamy
Sep 15, 1987·European Journal of Biochemistry·Z Wasylewski, M R Eftink
Mar 15, 1988·European Journal of Biochemistry·Z WasylewskiA Wasniowska
Dec 15, 1988·European Journal of Biochemistry·Z WasylewskiW Stryjewski
May 15, 1992·European Journal of Biochemistry·Z WasylewskiK Slizowska
Aug 15, 1992·European Journal of Biochemistry·D T DrydenR H Pain
Jul 1, 1981·Analytical Biochemistry·M R Eftink, C A Ghiron
Oct 25, 2000·Photochemistry and Photobiology·J R Lakowicz
Oct 6, 2006·The Biochemical Journal·Tirumala Kumar ChowdaryCh Mohan Rao
Dec 1, 1991·Biophysical Chemistry·J M JanotD Phillips
Mar 1, 1994·Biophysical Chemistry·Y C Chang, R D Ludescher
Sep 7, 1984·Biochimica Et Biophysica Acta·P MidouxM Monsigny
Oct 13, 2009·Journal of Photochemistry and Photobiology. B, Biology·Atul KumarArchana Pundle

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.