Time-resolved structural studies with serial crystallography: A new light on retinal proteins

Structural Dynamics
Valerie PanneelsGebhard Schertler

Abstract

Structural information of the different conformational states of the two prototypical light-sensitive membrane proteins, bacteriorhodopsin and rhodopsin, has been obtained in the past by X-ray cryo-crystallography and cryo-electron microscopy. However, these methods do not allow for the structure determination of most intermediate conformations. Recently, the potential of X-Ray Free Electron Lasers (X-FELs) for tracking the dynamics of light-triggered processes by pump-probe serial femtosecond crystallography has been demonstrated using 3D-micron-sized crystals. In addition, X-FELs provide new opportunities for protein 2D-crystal diffraction, which would allow to observe the course of conformational changes of membrane proteins in a close-to-physiological lipid bilayer environment. Here, we describe the strategies towards structural dynamic studies of retinal proteins at room temperature, using injector or fixed-target based serial femtosecond crystallography at X-FELs. Thanks to recent progress especially in sample delivery methods, serial crystallography is now also feasible at synchrotron X-ray sources, thus expanding the possibilities for time-resolved structure determination.

References

Oct 18, 1991·Science·R W SchoenleinC V Shank
Apr 22, 1993·Nature·G F SchertlerR Henderson
Aug 5, 2000·Science·K PalczewskiM Miyano
Nov 25, 2000·Biochimica Et Biophysica Acta·J RigaudJ Ranck
Nov 14, 2001·Biochemistry·J E KimR A Mathies
Aug 26, 2004·Journal of Molecular Biology·Tetsuji OkadaVolker Buss
Sep 29, 2004·Journal of Structural Biology·Friedrich SchottePhilip A Anfinrud
Oct 20, 2004·Journal of Molecular Biology·Jade LiGebhard F X Schertler
Jul 27, 2005·Current Opinion in Structural Biology·Gebhard F X Schertler
Sep 1, 2005·Current Opinion in Structural Biology·Dominique Bourgeois, Antoine Royant
Dec 22, 2005·Acta Crystallographica. Section D, Biological Crystallography·Elspeth F Garman, Robin Leslie Owen
Apr 6, 2006·Angewandte Chemie·Hitoshi Nakamichi, Tetsuji Okada
Aug 16, 2006·Proceedings of the National Academy of Sciences of the United States of America·Hitoshi Nakamichi, Tetsuji Okada
Sep 11, 2007·Journal of Molecular Biology·Jörg StandfussGebhard F X Schertler
Jun 20, 2008·Nature·Jung Hee ParkOliver Peter Ernst
Sep 27, 2008·Nature·Patrick ScheererOliver P Ernst
Oct 29, 2008·Journal of Synchrotron Radiation·D A ShapiroU Weierstall
Apr 8, 2009·Journal of the American Chemical Society·Víctor A Lórenz-Fonfría, Hideki Kandori
Aug 17, 2010·Neuropharmacology·Hui-Woog ChoeOliver P Ernst
Oct 5, 2010·Methods in Enzymology·Priyanka D AbeyrathneHenning Stahlberg
Feb 5, 2011·Nature·Henry N ChapmanJohn C H Spence
Mar 11, 2011·Nature·Jörg StandfussGebhard F X Schertler
Apr 8, 2011·Annual Review of Analytical Chemistry·David J KissickGarth J Simpson
Dec 27, 2011·Proceedings of the National Academy of Sciences of the United States of America·Xavier DeupiJörg Standfuss
Feb 15, 2012·Optics Express·Andrew AquilaHenry N Chapman
Feb 22, 2012·Acta Crystallographica. Section D, Biological Crystallography·Arash Zarrine-AfsarR J Dwayne Miller
Apr 3, 2012·The Review of Scientific Instruments·U WeierstallR B Doak
Oct 24, 2012·Acta Crystallographica. Section D, Biological Crystallography·Raymond G SierraMichael J Bogan
Jan 23, 2013·EMBO Molecular Medicine·Peter Hegemann, Georg Nagel
Mar 23, 2013·Frontiers in Molecular Neuroscience·Alexandre MourotRichard H Kramer
Sep 17, 2013·Angewandte Chemie·Jung Hee ParkOliver P Ernst
Dec 21, 2013·Science·Wei LiuVadim Cherezov
Dec 21, 2013·Methods in Molecular Biology·Kenneth N GoldieHenning Stahlberg
Dec 25, 2013·Chemical Reviews·Oliver P ErnstHideki Kandori
Feb 15, 2014·Nature Communications·Uwe WeierstallVadim Cherezov
Jun 11, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Bill PedriniXiao-Dan Li
Jul 31, 2014·IUCrJ·Matthias FrankJames E Evans
Aug 13, 2014·Scientific Reports·Mark S HunterMatthias Frank

❮ Previous
Next ❯

Citations

Mar 17, 2018·Biochemistry. Biokhimii︠a︡·G K SelikhanovA G Gabdulkhakov
Nov 20, 2016·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·Hideaki E KatoOsamu Nureki
May 2, 2018·Journal of Synchrotron Radiation·Andreas StreunAlbin Wrulich
Jan 30, 2018·Structural Dynamics·Rafael AbelaLuc Patthey
May 8, 2021·The Journal of Biological Chemistry·Mitchell D Miller, George N Phillips

❮ Previous
Next ❯

Methods Mentioned

BETA
X-ray
2D
2D crystallography

Software Mentioned

SFX
TR

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.