Time-resolved synchrotron radiation X-ray solution scattering study of DNA melting

Biopolymers
J PuigdomenechM H Koch

Abstract

Time resolved x-ray solution scattering measurements were made during thermal denaturation of DNA from various sources in the temperature range of 20-90 degrees C. Preliminary results on the influence of fragment length, ionic strength, and origin of the DNA on the time course of the scattering are described. Interpretation is based on model calculations of the scattering patterns. The results indicate that, for long DNA fragments at very low ionic strength, the melting process is a continuous phenomenon over the whole temperature range. It is accompanied by a progressive decrease of the radius of gyration of the cross section and of the mass per unit length. For short fragments of 146 base pair nucleosomal core DNA, stiffening of the DNA appears to precede a sharp melting transition.

References

Jan 1, 1982·Chromosoma·L P GrauJ A Subirana

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Citations

Sep 1, 1994·Biopolymers·N I KorolevI A Kuznetsov
May 29, 2012·Biomacromolecules·Cencen ZhangHesun Zhu
Nov 25, 2011·The European Physical Journal. E, Soft Matter·V MengarelliM Zeghal
Apr 5, 2017·Annual Review of Physical Chemistry·Hosung KiHyotcherl Ihee

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