Abstract
PixD (Slr1694) is a blue light receptor that contains a BLUF (blue light sensors using a flavin chromophore) domain. A protein-protein interaction between PixD and a response regulator PixE (Slr1693) is essential to achieve light signal transduction for phototaxis of the species. Although the initial photochemical reaction of PixD, the red shift of the flavin absorption spectrum, has been investigated, the subsequent reaction dynamics remain largely unresolved. Only the disassembly of the PixD(10)-PixE(5) dark complex has been characterized by static size exclusion chromatography. In this report, interprotein reaction dynamics were examined using time-resolved transient grating spectroscopy. The dissociation process was clearly observed as the light-induced diffusion coefficient change in the time domain, and the kinetics was determined. More strikingly, disassembly was found to take place only after photoactivation of two PixD subunits in the complex. This result suggests that the biological response of PixD does not follow a linear correlation with the light intensity but appears to be light-intensity-dependent.
References
Jan 22, 1998·Plant & Cell Physiology·T Kaneko, S Tabata
Oct 9, 2002·Trends in Biochemical Sciences·Mark Gomelsky, Gabriele Klug
Jan 21, 2004·Accounts of Chemical Research·Michael A van der Horst, Klaas J Hellingwerf
Nov 6, 2004·FEBS Letters·Shinji Masuda, Taka-aki Ono
Nov 24, 2004·Biochemistry·Koji HasegawaTaka-aki Ono
Jan 22, 2005·Plant & Cell Physiology·Koji HasegawaTaka-Aki Ono
Jul 9, 2005·Journal of Biochemistry·Koji OkajimaMasahiko Ikeuchi
Jul 11, 2006·Proceedings of the National Academy of Sciences of the United States of America·Magdalena GaudenJohn T M Kennis
Oct 18, 2006·Biochemistry·Hua YuanCarl Bauer
Oct 26, 2007·Current Opinion in Structural Biology·John T M Kennis, Marie-Louise Groot
Nov 22, 2007·Photochemistry and Photobiology·Aba Losi
Apr 11, 2008·Current Opinion in Microbiology·Erin B Purcell, Sean Crosson
Aug 13, 2008·Proceedings of the National Academy of Sciences of the United States of America·Hua Yuan, Carl E Bauer
Aug 19, 2008·Biophysical Journal·Cosimo BonettiJohn T M Kennis
Mar 3, 2010·Annual Review of Plant Biology·Andreas MöglichKeith Moffat
May 3, 2011·Journal of Molecular Biology·Keisuke TanakaMasahide Terazima
Jun 22, 2011·Biochemistry·Hua YuanCarl E Bauer
Citations
Dec 18, 2012·Plant & Cell Physiology·Shinji Masuda
Feb 11, 2014·Interface Focus·John T M Kennis, Tilo Mathes
May 20, 2015·FEBS Letters·Shukun RenShinji Masuda
Nov 19, 2015·Frontiers in Molecular Biosciences·Tilo Mathes, Jan P Götze
Aug 1, 2012·Plant & Cell Physiology·Shukun RenShinji Masuda
Dec 8, 2016·Photochemistry and Photobiology·Masato Kondoh, Masahide Terazima
Dec 29, 2016·Plant & Cell Physiology·Yuki SugimotoShinji Masuda
May 10, 2017·Integrative Biology : Quantitative Biosciences From Nano to Macro·Guangsheng PeiWeiwen Zhang
Mar 9, 2018·Physical Chemistry Chemical Physics : PCCP·Kosei ShibataMasahide Terazima
Oct 28, 2017·FEMS Microbiology Reviews·Annegret Wilde, Conrad W Mullineaux
Dec 14, 2017·Biophysics Reviews·Tomotsumi Fujisawa, Shinji Masuda
Aug 4, 2018·Antioxidants & Redox Signaling·Emma SevillaMaría F Fillat
Oct 18, 2019·Plant & Cell Physiology·Annik JakobShinji Masuda
Jun 7, 2017·Proceedings of the National Academy of Sciences of the United States of America·Daisuke Nakane, Takayuki Nishizaka
Mar 9, 2017·MBio·Rosanna Man Wah ChauKerwyn Casey Huang
May 18, 2018·Scientific Reports·Marisel R TuttobeneMaría Alejandra Mussi
Apr 8, 2020·Frontiers in Microbiology·Mengliang ShiWeiwen Zhang
Mar 29, 2013·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Kunisato KuroiMasahide Terazima
Oct 30, 2020·Life·Fabian D ConradiAnnegret Wilde
Jun 22, 2020·Current Opinion in Biotechnology·Monireh MarsafariPeng Xu
Apr 23, 2021·Accounts of Chemical Research·Masahide Terazima
Aug 26, 2014·The Journal of Physical Chemistry. B·Florimond ColletteMarcel Schmidt am Busch
Mar 9, 2019·The Journal of Physical Chemistry. B·Yusuke NakasoneMasahide Terazima