Time-resolved tracking of interprotein signal transduction: Synechocystis PixD-PixE complex as a sensor of light intensity

Journal of the American Chemical Society
Keisuke TanakaMasahide Terazima

Abstract

PixD (Slr1694) is a blue light receptor that contains a BLUF (blue light sensors using a flavin chromophore) domain. A protein-protein interaction between PixD and a response regulator PixE (Slr1693) is essential to achieve light signal transduction for phototaxis of the species. Although the initial photochemical reaction of PixD, the red shift of the flavin absorption spectrum, has been investigated, the subsequent reaction dynamics remain largely unresolved. Only the disassembly of the PixD(10)-PixE(5) dark complex has been characterized by static size exclusion chromatography. In this report, interprotein reaction dynamics were examined using time-resolved transient grating spectroscopy. The dissociation process was clearly observed as the light-induced diffusion coefficient change in the time domain, and the kinetics was determined. More strikingly, disassembly was found to take place only after photoactivation of two PixD subunits in the complex. This result suggests that the biological response of PixD does not follow a linear correlation with the light intensity but appears to be light-intensity-dependent.

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Citations

Dec 18, 2012·Plant & Cell Physiology·Shinji Masuda
Feb 11, 2014·Interface Focus·John T M Kennis, Tilo Mathes
Nov 19, 2015·Frontiers in Molecular Biosciences·Tilo Mathes, Jan P Götze
Mar 9, 2018·Physical Chemistry Chemical Physics : PCCP·Kosei ShibataMasahide Terazima
Oct 28, 2017·FEMS Microbiology Reviews·Annegret Wilde, Conrad W Mullineaux
Dec 14, 2017·Biophysics Reviews·Tomotsumi Fujisawa, Shinji Masuda
Aug 4, 2018·Antioxidants & Redox Signaling·Emma SevillaMaría F Fillat
Jun 7, 2017·Proceedings of the National Academy of Sciences of the United States of America·Daisuke Nakane, Takayuki Nishizaka
Mar 9, 2017·MBio·Rosanna Man Wah ChauKerwyn Casey Huang
Mar 29, 2013·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Kunisato KuroiMasahide Terazima
Jun 22, 2020·Current Opinion in Biotechnology·Monireh MarsafariPeng Xu
Aug 26, 2014·The Journal of Physical Chemistry. B·Florimond ColletteMarcel Schmidt am Busch
Mar 9, 2019·The Journal of Physical Chemistry. B·Yusuke NakasoneMasahide Terazima

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