Time-Resolved X-Ray Solution Scattering Reveals the Structural Photoactivation of a Light-Oxygen-Voltage Photoreceptor.

Structure
Oskar BerntssonSebastian Westenhoff

Abstract

Light-oxygen-voltage (LOV) receptors are sensory proteins controlling a wide range of organismal adaptations in multiple kingdoms of life. Because of their modular nature, LOV domains are also attractive for use as optogenetic actuators. A flavin chromophore absorbs blue light, forms a bond with a proximal cysteine residue, and induces changes in the surroundings. There is a gap of knowledge on how this initial signal is relayed further through the sensor to the effector module. To characterize these conformational changes, we apply time-resolved X-ray scattering to the homodimeric LOV domain from Bacillus subtilis YtvA. We observe a global structural change in the LOV dimer synchronous with the formation of the chromophore photoproduct state. Using molecular modeling, this change is identified as splaying apart and relative rotation of the two monomers, which leads to an increased separation at the anchoring site of the effector modules.

Citations

Jun 2, 2018·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Dolev RimmermanLin X Chen
Jul 17, 2018·PloS One·Katrin RöllenAndreas Maximilian Stadler
Aug 10, 2019·Protein Science : a Publication of the Protein Society·Andreas Möglich
Nov 28, 2019·Physical Chemistry Chemical Physics : PCCP·Kunisato KuroiTakakazu Nakabayashi
Aug 19, 2017·Nature Communications·Oskar BerntssonSebastian Westenhoff
May 10, 2020·Journal of Synchrotron Radiation·Jungmin KimHyotcherl Ihee
Jul 17, 2018·Nature Reviews. Microbiology·Françoise Jacob-DubuissonRudy Antoine
Jun 22, 2019·Annual Review of Microbiology·Alejandro Buschiazzo, Felipe Trajtenberg
Apr 18, 2021·The FEBS Journal·Harshwardhan PoddarNigel S Scrutton
Jun 28, 2021·Current Opinion in Structural Biology·Hyun Sun ChoPhilip Anfinrud
Jul 16, 2021·Journal of Photochemistry and Photobiology. B, Biology·Misato IkomaMasahide Terazima
Aug 4, 2021·Acta Crystallographica. Section D, Structural Biology·Andrea CelliniSebastian Westenhoff
Jul 10, 2018·Chemical Reviews·Aba LosiAndreas Möglich
Aug 31, 2017·The Journal of Physical Chemistry Letters·Dolev RimmermanLin X Chen
Jan 5, 2019·The Journal of Physical Chemistry. B·Kunitaka Ota, Takahisa Yamato
Aug 14, 2020·Biochemistry·L HenryS Westenhoff
Nov 9, 2021·The Journal of Physical Chemistry. B·Adam K NijhawanKevin L Kohlstedt

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Protein Structures (ASM)

Bacterial protein structures can expedite the development of novel antibiotics. Here is the latest research on bacterial proteins and the resolution of their structures.

Bacterial Protein Structures

Bacterial protein structures can expedite the development of novel antibiotics. Here is the latest research on bacterial proteins and the resolution of their structures.