Time series proteome profiling to study endoplasmic reticulum stress response.

Journal of Proteome Research
Michelle MintzYetrib Hathout

Abstract

Time series profiling is a powerful approach for obtaining information on protein expression dynamics and prevailing biochemical pathways. To date, such information could only be obtained at the mRNA level using mature and highly parallel technologies such as microarray gene expression profiling. The generation of time series data at the protein level has lagged due to the lack of robust and highly reproducible methodologies. Using a combination of SILAC strategy, SDS-PAGE and LC-MS/MS, we demonstrate successful monitoring of expression levels of the same set of proteins across different time points within the ER compartment of human primary fibroblast cells when exposed to ER stress inducers tunicamycin and thapsigargin. Data visualization was facilitated using GeneSpring GX analysis platform that was designed to process Affymetrix microarray data. This software also facilitated the generation of important parameters such as data normalization, calculation of statistical values to extract significant changes in protein expression, and the cross comparison of data sets.

References

Apr 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·O ThastrupA P Dawson
May 23, 2002·The Journal of Biological Chemistry·Po ZhaoEric P Hoffman
Jan 7, 2005·Nature·Jens S AndersenMatthias Mann
Mar 1, 2005·Journal of the American Society for Mass Spectrometry·Brian D HalliganAndrew S Greene
Oct 11, 2005·Journal of Proteome Research·Ramars AmanchyAkhilesh Pandey
Jan 18, 2006·Analytical Chemistry·John R YatesVlad Zabrouskov
Jun 21, 2006·Proceedings of the National Academy of Sciences of the United States of America·Ron BosePhilip A Cole
Dec 26, 2006·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Takeaki FukudaToshimitsu Suzuki
Mar 29, 2007·Proceedings of the National Academy of Sciences of the United States of America·Alejandro Wolf-YadlinForest M White
May 9, 2007·Methods in Molecular Biology·Shao-En Ong, Matthias Mann
Jun 15, 2007·Nature Reviews. Molecular Cell Biology·David Ron, Peter Walter
Jul 24, 2007·Molecular & Cellular Proteomics : MCP·Débora BonenfantJan van Oostrum
Oct 2, 2007·Journal of Proteome Research·Steven SwatkoskiCatherine Fenselau
Feb 15, 2008·Journal of Proteome Research·Stefan HankeMatthias Mann

❮ Previous
Next ❯

Citations

Jul 4, 2009·Amino Acids·Jens Allmer
Sep 24, 2010·The Journal of Biological Chemistry·Yajuan ZhuPeter Cresswell
Dec 17, 2009·Antioxidants & Redox Signaling·Miklós CsalaGábor Bánhegyi
Jun 18, 2011·Expert Review of Proteomics·Jatin G Burniston, Eric P Hoffman
Feb 26, 2016·European Journal of Cell Biology·Kateřina KratochvílováPetr Vaňhara
Aug 8, 2015·American Journal of Respiratory Cell and Molecular Biology·Jiawei JinRunlin Z Ma
Jun 29, 2012·Electrophoresis·Vibeke Hervik Bull, Bernd Thiede
Feb 11, 2011·Journal of Cellular Biochemistry·Ashleigh E NugentWalter E Horton
Mar 3, 2010·Immunological Reviews·Robert A ColbertGerlinde Layh-Schmitt
Sep 10, 2016·Aquatic Toxicology·Allison Kupsco, Daniel Schlenk
Feb 26, 2014·BMC Bioinformatics·Paul CraigJessie Kennedy
Oct 28, 2019·Proteomics·Alberto Valdés, Sara Bergström Lind
Oct 23, 2020·Bioinformatics and Biology Insights·Deepak MavRichard S Paules

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.