Time window and pharmacological characterisation of kainate-mediated preconditioning in organotypic rat hippocampal slice cultures

Neuroscience Letters
Rose NvueAshley K Pringle

Abstract

Tolerance to normally neurotoxic insults can be induced by prior a preconditioning exposure to a sublethal insult. Kainate toxicity can be attenuated by prior exposure to very low concentrations of kainate both in vivo and in vitro. Using organotypic hippocampal slice cultures from rats we have shown that 5 microM kainate induces a selective lesion in the CA3 region and this can be significantly attenuated by 1 microM kainate administered 1-5 days earlier. The time window for this effect was affected by the length of time in culture, and preconditioning was blocked by NBQX but not the selective AMPA receptor antagonist GYKI53655. These data demonstrate a role for kainate receptors in preconditioning for the first time and show that organotypic cultures can be used as a model to investigate long-term preconditioning mechanisms.

Citations

Jul 19, 2006·Cell and Tissue Research·Paulo Pinheiro, Christophe Mulle
Apr 20, 2005·Neurobiology of Disease·Venceslas DuveauGildas Le Gal La Salle
Nov 27, 2008·Alcoholism, Clinical and Experimental Research·Michael A CollinsRonald J Korthuis
May 16, 2008·Human & Experimental Toxicology·Mark P Mattson
Aug 28, 2007·Journal of Neuroscience Research·Barbara MioduszewskaLeszek Kaczmarek

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