Timing and targeting of P-element local transposition in the male germline cells of Drosophila melanogaster.

Genetics
Benjamin TimakovPing Zhang

Abstract

The P element in Drosophila melanogaster preferentially transposes into nearby sites. The local insertions display a preferential orientation toward the starting element. We investigated the mechanism of the P-element local transposition by isolating and characterizing local insertions in the male germline. We designed a genetic screen employing a marker gene that is carried in the P element and is dose sensitive. This dose effect allows isolation of flies containing newly transposed P elements in the presence of the starting element. A rapid molecular screen with PCR was used to identify 45 local insertions located within an approximately 40-kb genomic region on both sides of the starting element. Our system permits the isolation of the cluster progeny derived from a single insertion event, but none was isolated. The data suggest that local transposition occurs in the meiotic cell cycle. Nearly all of the local insertions were located within the promoter regions of the genes that were active in the male germline cells, suggesting that local insertions target predominantly active promoters. Our analysis shows that local transposition of the P element is highly regulated, displaying a cell-type specificity and a target specificity.

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Citations

Oct 13, 2006·PLoS Genetics·Jean-Claude WalserMartin E Feder
Jul 10, 2004·Proceedings of the National Academy of Sciences of the United States of America·Avril Coghlan, Kenneth H Wolfe
Aug 28, 2004·The Journal of Biological Chemistry·Geneviève MorrowRobert M Tanguay
Jun 2, 2005·Insect Molecular Biology·H MontenegroG D D Hurst
May 31, 2007·Journal of Biosciences·M B Evgen'evO G Zatsepina
Jun 5, 2007·Biochimica Et Biophysica Acta·Stanton B Gelvin, Sang-Ic Kim
Aug 14, 2003·Insect Biochemistry and Molecular Biology·Raymond WilsonDavid A O'Brochta
Jan 10, 2003·Molecular Biology and Evolution·Daniel N LermanMartin E Feder
Mar 17, 2004·Molecular Biology of the Cell·Anindya Ghosh-RoyKrishanu Ray
Feb 23, 2021·Biological Reviews of the Cambridge Philosophical Society·Roberta BergeroSimone Immler
Jan 28, 2003·Annals of the New York Academy of Sciences·Richard von Sternberg

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