Timing-dependent actions of NGF required for cell differentiation.

PloS One
Jaehoon ChungShinya Kuroda

Abstract

Continuous NGF stimulation induces PC12 cell differentiation. However, why continuous NGF stimulation is required for differentiation is unclear. In this study, we investigated the underlying mechanisms of the timing-dependent requirement of NGF action for cell differentiation. To address the timing-dependency of the NGF action, we performed a discontinuous stimulation assay consisting of a first transient stimulation followed by an interval and then a second sustained stimulation and quantified the neurite extension level. Consequently, we observed a timing-dependent action of NGF on cell differentiation, and discontinuous NGF stimulation similarly induced differentiation. The first stimulation did not induce neurite extension, whereas the second stimulation induced fast neurite extension; therefore, the first stimulation is likely required as a prerequisite condition. These observations indicate that the action of NGF can be divided into two processes: an initial stimulation-driven latent process and a second stimulation-driven extension process. The latent process appears to require the activities of ERK and transcription, but not PI3K, whereas the extension-process requires the activities of ERK and PI3K, but not transcript...Continue Reading

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Datasets Mentioned

BETA
GSE18016

Methods Mentioned

BETA
PCR
Feature Extraction

Software Mentioned

GOEAST
Image J
Agilent Feature Extraction
NeuroTracer
GeneSpring GX
Primer Express

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