DOI: 10.1101/197103Oct 2, 2017Paper

Timing of gene expression in a cell-fate decision system

BioRxiv : the Preprint Server for Biology
Delphine AymozSerge Pelet

Abstract

During development, morphogens provide extracellular cues allowing cells to select a specific fate by inducing complex transcriptional programs. The mating pathway in budding yeast offers simplified settings to understand this process. Pheromone secreted by the mating partner triggers the activity of a MAPK pathway, which results in the expression of hundreds of genes. Using a dynamic expression reporter, we quantified the kinetics of gene expression in single cells upon exogenous pheromone stimulation and in the physiological context of mating. In both conditions, we observed striking differences in the timing of induction of mating-responsive promoters. Biochemical analyses and generation of synthetic promoter variants demonstrated how the interplay between transcription factor binding and nucleosomes contribute to determine the kinetics of transcription in a simplified cell-fate decision system.

Related Concepts

Related Feeds

BioRxiv & MedRxiv Preprints

BioRxiv and MedRxiv are the preprint servers for biology and health sciences respectively, operated by Cold Spring Harbor Laboratory. Here are the latest preprint articles (which are not peer-reviewed) from BioRxiv and MedRxiv.

Related Papers

Molecular Systems Biology
Delphine AymozSerge Pelet
BioRxiv : the Preprint Server for Biology
Eric Durandau, Serge Pelet
Current Opinion in Immunology
H Clevers, P Ferrier
Tanpakushitsu kakusan koso. Protein, nucleic acid, enzyme
K IrieK Matsumoto
© 2021 Meta ULC. All rights reserved