Timing of perialgal vacuole membrane differentiation from digestive vacuole membrane in infection of symbiotic algae Chlorella vulgaris of the ciliate Paramecium bursaria

Protist
Yuuki Kodama, Masahiro Fujishima

Abstract

Each symbiotic Chlorella of the ciliate Paramecium bursaria is enclosed in a perialgal vacuole derived from the host digestive vacuole to protect from lysosomal fusion. To understand the timing of differentiation of the perialgal vacuole from the host digestive vacuole, algae-free P. bursaria cells were fed symbiotic C. vulgaris cells for 1.5min, washed, chased and fixed at various times after mixing. Acid phosphatase activity in the vacuoles enclosing the algae was detected by Gomori's staining. This activity appeared in 3-min-old vacuoles, and all algae-containing vacuoles demonstrated activity at 30min. Algal escape from these digestive vacuoles began at 30min by budding of the digestive vacuole membrane into the cytoplasm. In the budded membrane, each alga was surrounded by a Gomori's thin positive staining layer. The vacuoles containing a single algal cell moved quickly to and attached just beneath the host cell surface. Such vacuoles were Gomori's staining negative, indicating that the perialgal vacuole membrane differentiates soon after the algal escape from the host digestive vacuole. This is the first report demonstrating the timing of differentiation of the perialgal vacuole membrane during infection of P. bursaria wi...Continue Reading

Citations

Feb 4, 2010·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Eva C M Nowack, Michael Melkonian
Feb 19, 2015·Mitochondrial DNA. Part A. DNA Mapping, Sequencing, and Analysis·Massimiliano OrsiniGiacomo Cao
Dec 14, 2011·European Journal of Protistology·Masahiro Fujishima, Yuuki Kodama
Nov 11, 2014·The Journal of Animal Ecology·Aabir BanerjiOliver Kaltz
Jan 23, 2013·Journal of Microbiological Methods·Undine E M Achilles-Day, John G Day
Apr 27, 2011·The Journal of Eukaryotic Microbiology·Matthew D Johnson
Dec 1, 2021·Environmental Microbiology Reports·Kenta WatanabeMasahisa Watarai

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