Timing of the initial muscle biopsy does not affect the measured muscle protein fractional synthesis rate during basal, postabsorptive conditions.

Journal of Applied Physiology
Gordon I SmithB Mittendorfer

Abstract

The muscle protein fractional synthesis rate (FSR) is determined by monitoring the incorporation of an amino acid tracer into muscle protein during a constant-rate intravenous tracer infusion. Commonly two sequential muscle biopsies are obtained some time after starting the tracer infusion. However, other protocols, including those with an initial biopsy before starting the tracer infusion to measure the background enrichment and those with only a single biopsy after several hours of tracer infusion have been used. To assess the validity of these approaches, we compared the muscle protein FSR obtained by calculating the difference in [ring-(2)H(5)]phenylalanine and [5,5,5-(2)H(3)]leucine incorporation into muscle protein at approximately 3.5 h after starting the tracer infusion and 1) at 60 min; 2) before starting the tracer infusion (background enrichment); 3) a population average muscle protein background enrichment; and 4) by measuring the tracer incorporation into muscle protein at approximately 3.5 h assuming essentially no background enrichment. Irrespective of the tracer used, the muscle protein FSR calculated from the difference in the muscle protein labeling several hours after starting the tracer infusion and either t...Continue Reading

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Citations

Feb 27, 2013·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Meghann J RobinsonStuart M Phillips
May 27, 2011·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Matthew M RobinsonBenjamin F Miller
Nov 27, 2010·Journal of Applied Physiology·Gordon I SmithBettina Mittendorfer
Jan 17, 2012·Medicine and Science in Sports and Exercise·Gordon I SmithBettina Mittendorfer
Sep 9, 2010·Metabolism: Clinical and Experimental·Matthew P HarberTodd A Trappe
Jan 3, 2012·Metabolism: Clinical and Experimental·Nicholas A BurdLuc J C van Loon
Feb 9, 2012·The British Journal of Nutrition·Yifan YangStuart M Phillips
Nov 12, 2015·PloS One·Bart B L GroenLuc J C van Loon
Mar 3, 2012·Journal of Applied Physiology·Daniel W D WestStuart M Phillips
Jul 21, 2012·Journal of Applied Physiology·Nicholas A BurdLuc J C van Loon

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