TIMP-2 growth-stimulatory activity: a concentration- and cell type-specific response in the presence of insulin

Experimental Cell Research
J A NemethC L Goolsby

Abstract

In addition to proteinase-inhibitory activities, growth-stimulatory activities have been described for all three known members of the tissue inhibitors of the metalloproteinase (TIMP) family, TIMP-1, TIMP-2, and ChIMP-3, believed to be the chicken homologue of TIMP-3. However, the mechanism by which the TIMPs stimulate cell growth is unclear. In this report we have demonstrated that rTIMP-2 was growth-stimulatory for human foreskin fibroblasts (HSF4, HSF43, HS68), lung adenocarcinoma cells (A549), human melanoma cells (WM115), and the Burkitt's lymphoma cell line RAMOS, and this stimulatory response was concentration-dependent, with the greatest stimulation occurring a 10-30 pM rTIMP-2 in [3H]thymidine incorporation assays and at 20-100 pM in cell growth assays. Normal human colon (18Co) and lung (37Lu) fibroblasts showed no response to rTIMP-2. [3H]Thymidine incorporation was inhibited by rTIMP-2 treatment in the nonadherent cell line HL60. These studies also demonstrated that for the cell types tested, TIMP-2 alone was insufficient for a growth stimulatory response requiring, at a minimum, the presence of insulin. In the absence of any "co-factor(s)," such as insulin, TIMP-2 treatment was inhibitory.

Citations

Feb 7, 2001·Journal of the National Cancer Institute·M Hidalgo, S G Eckhardt
Nov 24, 2004·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·M MäättäT Turpeenniemi-Hujanen
Jan 1, 1997·Critical Reviews in Biochemistry and Molecular Biology·J T PriceE C Kohn
Jan 5, 2002·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·T SobueT Hayakawa
Aug 22, 2000·The Journal of Investigative Dermatology·U B HofmannD J Ruiter
Mar 4, 2008·Urologic Oncology·Mark Nogueira, Hyung L Kim
Oct 31, 2003·Molecular Carcinogenesis·Sonal K PatelSerrine S Lau
Nov 25, 2003·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·L NakopoulouA Keramopoulos
Oct 25, 2011·International Journal of Cancer. Journal International Du Cancer·Lan-Ping DiaoYu-Huan Gao
May 19, 2004·The International Journal of Biochemistry & Cell Biology·Floria LizárragaJorge Meléndez-Zajgla
May 24, 2005·Biochimica Et Biophysica Acta·Mikael Björklund, Erkki Koivunen
Jul 25, 2009·Journal of Molecular Signaling·Jun Sun, William G Stetler-Stevenson
Sep 29, 2001·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·T HagemannC Binder
Apr 6, 1999·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·P HenrietY A Declerck
Nov 10, 1998·International Journal of Cancer. Journal International Du Cancer·K J GossL M Matrisian
May 5, 2012·International Braz J Urol : Official Journal of the Brazilian Society of Urology·Sabrina Thalita ReisKatia Ramos Moreira Leite
Feb 24, 2001·Thyroid : Official Journal of the American Thyroid Association·Z Kraiem, S Korem
Aug 6, 2010·Cellular and Molecular Life Sciences : CMLS·Eleonore Fröhlich
Aug 24, 2010·Anti-cancer Drugs·Alvaro PintoEnrique Espinosa
Jan 18, 2002·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Lydia NakopoulouAntonios Keramopoulos
Oct 17, 2007·Molecular and Cellular Biochemistry·Judit MarsillachJorge Joven
Jun 12, 2001·Journal of Neuropathology and Experimental Neurology·A PagenstecherI L Campbell
Nov 10, 2004·Clinical Orthopaedics and Related Research·Diah S BramonoGregory H Altman
Sep 18, 2002·Journal of Cell Science·Andrew H BakerGillian Murphy
Oct 19, 2001·Biochemical and Biophysical Research Communications·T HoashiK Tamaki
Feb 15, 2001·Seminars in Cancer Biology·I Stamenkovic
Mar 23, 2005·Biochimie·Uta B HofmannJürgen C Becker

❮ Previous
Next ❯

Related Concepts

Related Feeds

B-Cell Lymphoma

B-cell lymphomas include lymphomas that affect B cells. This subtype of cancer accounts for over 80% of non-Hodgkin lymphomas in the US. Here is the latest research.