TiO2 nanoparticles tested in a novel screening whole human blood model of toxicity trigger adverse activation of the kallikrein system at low concentrations

Biomaterials
Barbro Ekstrand-HammarströmBo Nilsson

Abstract

There is a compelling need to understand and assess the toxicity of industrially produced nanoparticles (NPs). In order to appreciate the long-term effects of NPs, sensitive human-based screening tests that comprehensively map the NP properties are needed to detect possible toxic mechanisms. Animal models can only be used in a limited number of test applications and are subject to ethical concerns, and the interpretation of experiments in animals is also distorted by the species differences. Here, we present a novel easy-to-perform highly sensitive whole-blood model using fresh non-anticoagulated human blood, which most justly reflects complex biological cross talks in a human system. As a demonstrator of the tests versatility, we evaluated the toxicity of TiO2 NPs that are widely used in various applications and otherwise considered to have relatively low toxic properties. We show that TiO2 NPs at very low concentrations (50 ng/mL) induce strong activation of the contact system, which in this model elicits thromboinflammation. These data are in line with the finding of components of the contact system in the protein corona of the TiO2 NPs after exposure to blood. The contact system activation may lead to both thrombotic reacti...Continue Reading

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Citations

Mar 22, 2016·Environmental Technology·Marlon BrancherHenrique de Melo Lisboa
May 28, 2016·International Journal of Molecular Sciences·Jiangxue WangYubo Fan
Mar 10, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Igor RochaNatalia Ferraz
May 5, 2018·International Journal of Toxicology·Giray Buğra Akbaba, Hasan Türkez
Feb 14, 2018·Nanoscale·Stefán B GunnarssonTommy Cedervall
Nov 4, 2020·Materials·Fredric G SvenssonVadim G Kessler
May 1, 2021·Nanomaterials·Fredric G SvenssonKarin Fromell

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