Tiotropium bromide attenuates respiratory syncytial virus replication in epithelial cells

Respiration; International Review of Thoracic Diseases
Ken IesatoHiroshi Shirasawa

Abstract

Respiratory syncytial virus (RSV) infection could be related to airway inflammation as well as exacerbation of chronic obstructive pulmonary disease (COPD). Tiotropium bromide decreases the frequency of exacerbation in patients with COPD; however, the mechanisms of tiotropium bromide to reduce the chances of exacerbation have not been defined. One potential mechanism could be that tiotropium bromide protects against RSV infection in epithelial cells. To examine whether tiotropium bromide affects RSV replication in HEp-2 cells. The supernatant titer of RSV was calculated by methylcellulose plaque assay after RSV innoculation. Intracellular RSV and ICAM-1 mRNA were measured by PCR. Syncytium formation was observed by light microscopy. Intracellular RSV fusion protein and RhoA protein were detected by Western blot analysis. Furthermore, RhoA activity, ICAM-1 expression and inflammatory cytokines in cultured supernatant were measured by binding assay, immunofluorescence staining and ELISA, respectively. Tiotropium bromide decreased the supernatant titer of RSV, and it inhibited syncytium formation, RhoA activation and ICAM-1 expression. Moreover, it suppressed the production of IL-6 and IL-8 after RSV infection. The antiviral effec...Continue Reading

Citations

Jul 28, 2012·The European Respiratory Journal·Jadwiga A WedzichaTerence A R Seemungal
Jul 16, 2010·International Journal of Chronic Obstructive Pulmonary Disease·Ekaterina S GladyshevaRobert L Owens
Jul 25, 2014·Drugs·Rachid Berair, Christopher E Brightling
Nov 15, 2011·Respiratory Physiology & Neurobiology·Masanori AsadaMutsuo Yamaya
Aug 25, 2015·Pediatric Surgery International·Ayako TakenouchiHiroshi Shirasawa
Mar 27, 2016·The Journal of Pharmacology and Experimental Therapeutics·Hannes BucherKlaus J Erb
Dec 7, 2016·American Journal of Respiratory and Critical Care Medicine·Kai M BeehJadwiga A Wedzicha
Jan 25, 2021·The Journal of Allergy and Clinical Immunology·Alberto PapiGiorgio Walter Canonica
Jul 13, 2021·Frontiers in Physiology·François M CarlierCharles Pilette

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