TIPE2 inhibits GC via regulation of cell proliferation, apoptosis and inflammation.

Oncology Reports
Zhenhe LinZhongquan Qi

Abstract

Gastric cancer (GC), a type of gastric mucosal epithelium disease caused by common malignant tumors, has become a major threat to human health and survival. Tumor necrosis factor‑α‑induced protein‑8 like‑2 (TIPE2) is a negative immune regulatory factor that is selectively expressed in immune organs, immune cells and various epithelial cells and serves an important role in the maintenance of human physiological immune homeostasis. In our preliminary study, we found that the expression of TIPE2 was downregulated or absent in GC tissues compared with normal gastric mucosa tissues, indicating that TIPE2 may play a significant role in the development of GC. To clarify the role of TIPE2 in the progression of human GC and to elucidate the underlying mechanism, the association between TIPE2 and phosphatidylinositol 3‑kinase (PI3K)/AKT, the cell cycle, the caspase‑related apoptosis pathway and the NF‑κB signaling pathway were investigated through western blot and flow cytometric analysis. It was determined that TIPE2 inhibited GC cell proliferation mainly by reducing the expression of phosphorylated AKT and ERK, which caused subsequent inhibition of the PI3K‑AKT and Ras‑Raf‑MEK‑ERK1/2 signaling pathways. Additionally, we investigated th...Continue Reading

Citations

Jun 5, 2020·American Journal of Physiology. Lung Cellular and Molecular Physiology·Bingqing ShiPeng Gao

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Methods Mentioned

BETA
xenograft
reverse transcription-PCR
PCR
Assay
optical microscopy
flow cytometry
ELISA
transfection

Software Mentioned

ImageJ
ModFit
SPSS

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