Tissue- and fibre-specific modifications of insulin-signalling molecules in cardiac and skeletal muscle of diabetic rats

Clinical and Experimental Pharmacology & Physiology
Demiana EkladousLise Coderre

Abstract

1. Levels of insulin-signalling molecules are altered in streptozotocin (STZ)-induced diabetes, a model of Type 1 diabetes. However, the tissue-specific regulation of these changes and the effect of insulin supplementation on signalling molecule protein levels have not been well characterized. 2. In the present study, we evaluated the level of proximal insulin-signalling intermediates in the heart and in red and white gastrocnemius muscles of 2 week diabetic rats and diabetic rats supplemented with insulin. 3. Diabetes augmented levels of the insulin receptor and the p85 regulatory subunit of phosphatidylinositol 3-kinase in the red gastrocnemius, but not in the white gastrocnemius or the heart. Furthermore, diabetes reduced insulin receptor substrate-1 levels in both the red and white gastrocnemius, but not in the heart. Examination of the levels and basal activities of distal insulin-signalling intermediates (protein kinase B (PKB)/Akt, extracellular signal-regulated kinase (ERK) 1/2, p38 mitogen-activated protein kinase (MAPK)) also failed to reveal a specific pattern in these changes. Thus, diabetes reduced basal ERK1/2 and PKB/Akt phosphorylation in the heart and white gastrocnemius, respectively, whereas it augmented basa...Continue Reading

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Citations

Jul 14, 2010·Antioxidants & Redox Signaling·Jakob Vinten-JohansenKarin Przyklenk
Aug 21, 2012·PloS One·Laura NocitoJoan J Guinovart
Jul 14, 2012·Differentiation; Research in Biological Diversity·Zoe Redshaw, Paul T Loughna
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Nov 10, 2011·American Journal of Physiology. Endocrinology and Metabolism·Juha J HulmiHeikki Kainulainen

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