Tissue- and time-dependent transcription in Ixodes ricinus salivary glands and midguts when blood feeding on the vertebrate host

Scientific Reports
Michalis KotsyfakisJosé M C Ribeiro

Abstract

Ixodes ricinus is a tick that transmits the pathogens of Lyme and several arboviral diseases. Pathogens invade the tick midgut, disseminate through the hemolymph, and are transmitted to the vertebrate host via the salivary glands; subverting these processes could be used to interrupt pathogen transfer. Here, we use massive de novo sequencing to characterize the transcriptional dynamics of the salivary and midgut tissues of nymphal and adult I. ricinus at various time points after attachment on the vertebrate host. Members of a number of gene families show stage- and time-specific expression. We hypothesize that gene expression switching may be under epigenetic control and, in support of this, identify 34 candidate proteins that modify histones. I. ricinus-secreted proteins are encoded by genes that have a non-synonymous to synonymous mutation rate even greater than immune-related genes. Midgut transcriptome (mialome) analysis reveals several enzymes associated with protein, carbohydrate, and lipid digestion, transporters and channels that might be associated with nutrient uptake, and immune-related transcripts including antimicrobial peptides. This publicly available dataset supports the identification of protein and gene targe...Continue Reading

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Citations

Feb 13, 2016·Ticks and Tick-borne Diseases·Ben J MansAbdalla A Latif
Nov 2, 2015·Trends in Parasitology·Jindřich ChmelařMichail Kotsyfakis
Feb 3, 2016·Trends in Parasitology·Jindřich ChmelařMichail Kotsyfakis
Dec 26, 2015·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Xing-Li XuZhi-Hui Liao
Jul 21, 2015·Journal of Proteomics·Jan KotálJindřich Chmelař
Mar 29, 2016·Epigenetics : Official Journal of the DNA Methylation Society·Alejandro Cabezas-CruzJosé de la Fuente
Jun 25, 2015·Parasites & Vectors·Lucas TirloniItabajara Da Silva Vaz
May 15, 2015·PLoS Neglected Tropical Diseases·Michalis KotsyfakisJosé M C Ribeiro
Jul 18, 2016·Molecular Genetics and Genomics : MGG·Bo-Yin JiaFu-He Yang
Oct 4, 2016·Frontiers in Cellular and Infection Microbiology·José de la FuenteCatherine A Hill
Oct 18, 2016·Insect Biochemistry and Molecular Biology·Immacolata IovinellaFrancesca Romana Dani
Nov 9, 2016·Scientific Reports·Jan PernerPetr Kopáček
May 21, 2016·Parasite Immunology·J de la FuenteC Maritz-Olivier
Jan 12, 2016·PLoS Neglected Tropical Diseases·Tae Kwon KimAlbert Mulenga
Apr 26, 2017·Frontiers in Cellular and Infection Microbiology·José de la FuenteRyan O M Rego
Apr 14, 2018·PLoS Neglected Tropical Diseases·Jan PernerJosé M C Ribeiro
Dec 7, 2017·Frontiers in Cellular and Infection Microbiology·Eliane EstevesAndréa C Fogaça
Oct 11, 2017·Frontiers in Cellular and Infection Microbiology·Ben J MansRonel Pienaar
Jun 27, 2018·Parasites & Vectors·N Pierre CharrierClaude Rispe
Jul 25, 2019·Frontiers in Physiology·Iveta ŠtibrániováMária Kazimírová
Mar 7, 2020·Frontiers in Cellular and Infection Microbiology·Charles E HartSaravanan Thangamani
Jun 15, 2017·Frontiers in Cellular and Infection Microbiology·Jindřich ChmelařMichail Kotsyfakis
Jul 12, 2017·Parasites & Vectors·Elen AnatrielloBeatriz Rossetti Ferreira
Jul 27, 2017·Revista brasileira de parasitologia veterinária = Brazilian journal of veterinary parasitology : Órgão Oficial do Colégio Brasileiro de Parasitologia Veterinária·Gabriel Alves LandulfoDarci Moraes Barros-Battesti
Mar 6, 2019·BMC Genomics·Vera Margarete ScarpassaJosé M C Ribeiro
May 3, 2019·Scientific Reports·Ricardo N AraujoFabiano Oliveira
Nov 3, 2020·Parasite Immunology·Larissa Almeida MartinsMichail Kotsyfakis
Dec 29, 2020·Parasite Immunology·Jennifer D HelbleLinden T Hu
Feb 27, 2021·Frontiers in Immunology·Gabriel Cerqueira Alves CostaRicardo N Araujo
Mar 12, 2021·Ticks and Tick-borne Diseases·Tereza KozelkováPetr Kopáček

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Datasets Mentioned

BETA
SRR641331
GANP00000000
PRJNA217984

Software Mentioned

TopHat
samtools mpileup
BWA aln
samtools
R
Trans
Excel
Visual Basic
SOAPdenovo
blastn

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