Tissue and Exosomal Serine Protease Inhibitors Are Significantly Overexpressed in Chronic Rhinosinusitis With Nasal Polyps

American Journal of Rhinology & Allergy
Sarina K MuellerBenjamin S Bleier

Abstract

The fibrinolysis pathway has been previously implicated in the etiopathogenesis of chronic rhinosinusitis with nasal polyps (CRSwNP). The purpose of this study was (1) to explore protein derangements of selected protease inhibitors of the serpin superfamily in CRSwNP and (2) to correlate the protease inhibitor derangements of the fibrinolysis pathway in tissue with exosomal samples to evaluate the potential of an exosomal noninvasive "liquid biopsy" for CRSwNP. Institutional review board approved study in which matched tissue and mucus exosomal proteins (SerpinB2, SerpinF2, SerpinG1, and SerpinE1) were compared between control and CRSwNP patients using Western Blot analysis (n = 6/group) and immunohistochemistry (IHC). Transcriptome analysis (n = 10/group) on the same proteins was performed using whole transcriptome sequencing. Semiquantitative analysis of the Western Blots was performed using the Whitney-Mann U test. The transcriptomic data set showed multiple differentially expressed genes including SerpinB2 (fold changes [FC] 7.38), SerpinE1 (FC 1.42), SerpinF2 (FC 2.03), and SerpinG1 (FC 0.72). Western Blot and IHC analysis showed an overexpression of the Serpin protease inhibitors in tissue (SerpinB2, P < .01; SerpinE1, P ...Continue Reading

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Citations

Jul 17, 2019·American Journal of Rhinology & Allergy·Janalee Stokken
Jan 14, 2020·International Forum of Allergy & Rhinology·Sarina K MuellerBenjamin S Bleier
Feb 6, 2021·Diagnostics·Katarzyna PiszczatowskaMirosław J Szczepański

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Methods Mentioned

BETA
biopsies
biopsy

Software Mentioned

GraphPad
Ballgown
StringTie
HISAT27
VisionWorks
FactoMineR
Prism Prism
SAMtools

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