Tissue and intrahepatic distribution and subcellular localization of a mannosylated lipoplex after intravenous administration in mice

Journal of Controlled Release : Official Journal of the Controlled Release Society
Makiko YamadaMitsuru Hashida

Abstract

We have previously reported that, unlike a lipoplex and mannosylated (Man) lipoplex underwent gene transfer to liver nonparenchymal cells (NPC) that possess mannose receptors after intravenous administration in mice. In this study, the tissue, intrahepatic distribution, and subcellular localization of the lipoplex after intravenous administration were investigated. DC-Chol liposome was selected as a cationic liposomes. After administration of lipoplex and Man lipoplex, the high gene expression was observed in the lung and liver, respectively. After administration of [32P]Man lipoplex, about 80% of [32P]plasmid DNA (pDNA) was accumulated in the liver. As for the intrahepatic distribution, the NPC/parenchymal cells (PC) ratio of [32P]Man lipoplex was 9.64, whereas the NPC/PC ratio of [32P]lipoplex was 1.93. The radioactivity in the cytosolic fraction of liver homogenate of [111In]Man lipoplex was two-fold higher than that of [111In]lipoplex, indicating that Man liposomes facilitate the release of pDNA into the cytosolic space. However, a rapid sorting of the radioactivity from endosomes to lysosomes was observed with the [111In]Man lipoplex. Also, amplification of pDNA by PCR suggested that the Man lipoplex is more rapidly degrad...Continue Reading

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Citations

Apr 6, 2006·Biological & Pharmaceutical Bulletin·Takeshi TeradaMitsuru Hashida
Sep 2, 2009·Biological & Pharmaceutical Bulletin·Kazumi NakamuraMitsuru Hashida
Mar 7, 2012·International Journal of Nanomedicine·Fansheng KongYong Wang
May 24, 2012·International Journal of Nanomedicine·Zhaoshun JiangFansheng Kong
Jan 5, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Tomoaki KurosakiMitsuru Hashida
Dec 8, 2005·Journal of Drug Targeting·Wassana YeepraeMitsuru Hashida
Jun 6, 2008·Expert Opinion on Drug Delivery·Juan M IracheSocorro Espuelas
Nov 18, 2014·Expert Opinion on Biological Therapy·Hojat BornaSadegh Azimzadeh Jamalkandi
Jun 11, 2014·Journal of Controlled Release : Official Journal of the Controlled Release Society·Shigeru Kawakami, Mitsuru Hashida
Sep 27, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·Yoshiyuki HattoriMitsuru Hashida
Feb 3, 2007·Journal of Controlled Release : Official Journal of the Controlled Release Society·Kosuke ShigetaMitsuru Hashida
Apr 21, 2006·The Journal of Gene Medicine·Yoshiyuki HattoriMitsuru Hashida
Nov 6, 2012·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Keita Un
Aug 5, 2005·Chemical & Pharmaceutical Bulletin·Mitsuru HashidaFumiyoshi Yamashita
May 4, 2006·The Journal of Pharmacology and Experimental Therapeutics·Yoshiyuki HattoriMitsuru Hashida

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