Tissue and organ culture studies of hepatitis B virus.

The American Journal of the Medical Sciences
A J Zuckerman

Abstract

Techniques have been developed for the cultivation of human and nonhuman primate liver cells in tissue and organ culture. Progressive non-cytocidal involvement of the normal cytoplasmic and nuclear components of cultured liver cells has been demonstrated by specific attachment of fluorescent antibody to hepatitis B core and surface antigens after inoculation of the cultures of human origin with known infective sera and with clinical material. Hepatitis B surface antigen may be produced, although infrequently, in inoculated liver organ cultures but serial passage has not been achieved. Serial passage of hepatitis B virus has been reported with fragments of human embryo liver cultivated on the chorioallantoic membrane of the developing chick embryo. Intranuclear virus-like particles have been localized in hepatocytes of cultured explants of liver biopsies obtained from infants with chronic hepatitis B antigenemia. Further studies are urgently required to determine whether cultivation of hepatitis B virus can be firmly established in readily available cell and organ cultures.

Citations

Jan 1, 1980·Clinics in Gastroenterology·P J Jenkins, R Williams
Dec 1, 1978·The British Journal of Addiction to Alcohol and Other Drugs·D H Judson
Jun 1, 1978·Journal of Dentistry·J D LilleyJ A Waterhouse
Jul 12, 2001·Journal of Gastroenterology and Hepatology·R Schmid
Mar 1, 1979·Journal of Toxicology and Environmental Health·A J Zuckerman
Jan 1, 1981·Nutrition and Cancer·P O Okonkwo, C N Obionu
Oct 1, 1977·Vox Sanguinis·V T BabesG Buzernescu

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