PMID: 9443078Jan 27, 1998Paper

Tissue, cell type, and breast cancer stage-specific expression of a TGF-beta inducible early transcription factor gene

Journal of Cellular Biochemistry
Malayannan SubramaniamThomas Spelsberg

Abstract

This laboratory has previously identified a novel TGF-beta inducible early gene (TIEG) in human osteoblasts [Subramaniam et al. (1995): Nucleic Acids Res 23:4907-4912]. Using TIEG specific polyclonal antibody and immunoprecipitation methods in normal human fetal osteoblast cells (hFOB cells), we have now demonstrated that TIEG encodes a 72-kDa protein whose levels are transiently increased at as early as 2 h of TGF-beta treatment. Polarized confocal microscopic analysis of hFOB cells shows a nuclear localized TIEG protein in untreated cells under the conditions described under Methods. Interestingly, the levels of TIEG protein in the nuclei increase when the cells are treated with TGF-beta 1 for 2 h. In contrast, similar analyses of untreated human keratinocytes show a cytoplasmic localized TIEG protein that appears to be translocated to the nucleus after H2O2 treatment. Additional immunohistochemical studies have demonstrated that TIEG protein is expressed in epithelial cells of the placenta, breast, and pancreas, as well as in osteoblast cells of bone and selected other cells of the bone marrow and cerebellum with some cells showing a cytoplasmic localization and others a nuclear localization. All cells of the kidney display ...Continue Reading

Citations

May 17, 2011·Cell and Tissue Research·Björn Spittau, Kerstin Krieglstein
Jan 4, 2001·The International Journal of Biochemistry & Cell Biology·D T DangV W Yang
Jun 26, 2004·Breast Cancer Research and Treatment·Monica M ReinholzThomas C Spelsberg
Aug 24, 1999·Nucleic Acids Research·S Philipsen, G Suske
Jan 20, 2005·Molecular and Cellular Biology·Malayannan SubramaniamThomas C Spelsberg
Oct 19, 2010·Cellular and Molecular Life Sciences : CMLS·Endalkachew A AlemuTerje Johansen
Oct 21, 2010·Physiological Reviews·Beth B McConnell, Vincent W Yang
Sep 18, 2008·International Journal of Cancer. Journal International Du Cancer·Guri Døsen-DahlEdith Rian
Oct 8, 2004·Critical Reviews in Oral Biology and Medicine : an Official Publication of the American Association of Oral Biologists·Bernhard Ganss, Andrew Jheon

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