Tissue culture-induced genetic and epigenetic variation in triticale (× Triticosecale spp. Wittmack ex A. Camus 1927) regenerants

Plant Molecular Biology
Joanna MachczyńskaP T Bednarek

Abstract

Plant regeneration via in vitro culture can induce genetic and epigenetic variation; however, the extent of such changes in triticale is not yet understood. In the present study, metAFLP, a variation of methylation-sensitive amplified fragment length polymorphism analysis, was used to investigate tissue culture-induced variation in triticale regenerants derived from four distinct genotypes using androgenesis and somatic embryogenesis. The metAFLP technique enabled identification of both sequence and DNA methylation pattern changes in a single experiment. Moreover, it was possible to quantify subtle effects such as sequence variation, demethylation, and de novo methylation, which affected 19, 5.5, 4.5% of sites, respectively. Comparison of variation in different genotypes and with different in vitro regeneration approaches demonstrated that both the culture technique and genetic background of donor plants affected tissue culture-induced variation. The results showed that the metAFLP approach could be used for quantification of tissue culture-induced variation and provided direct evidence that in vitro plant regeneration could cause genetic and epigenetic variation.

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Citations

Aug 17, 2020·International Journal of Molecular Sciences·Piotr T BednarekRenata Orłowska
Mar 12, 2020·International Journal of Molecular Sciences·Xiaoru FanXiaorong Fan
Feb 13, 2020·Plant Molecular Biology·Renata Orłowska, Piotr Tomasz Bednarek
Feb 23, 2021·Frontiers in Plant Science·Renata OrłowskaPiotr T Bednarek
Mar 18, 2021·Journal of Biological Research·Renata Orłowska
Jul 25, 2021·International Journal of Molecular Sciences·Piotr Tomasz BednarekRenata Orłowska

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Software Mentioned

metAFLP
R CRAN
SAS
SAM
PAST

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