Tissue culture-induced somaclonal variation of decreased pollen viability in torenia (Torenia fournieri Lind.)

Botanical Studies
ShuLan SunXiaoJing Wang

Abstract

Phenotypic and genotypic variations, collectively called somaclonal variations, are induced during tissue culture. We studied the phenotypic variation in pollen viability of regenerants of torenia after subculturing for one to nine generations. We found that pollen viability of regenerants continuously decreased with increasing subculture time. High concentrations of plant growth regulators applied to the Murashige and Skoog (MS) medium also resulted in diminished pollen viability. Furthermore, antibiotic application during gene transformation also decreased pollen viability of the transformants. However, the process of long-term culture did not significantly change pollen viability. The mean methylation level of regenerants showed a 0.28% to 3.95% decrease in seedlings subcultured in vitro for nine generations. Moreover, when the ninth subcultured regenerants with reduced pollen vibility were recovered in soil to get seeds, the pollen viability of seed-derive plants was similar to that of the wild type. The results show that plant growth regulators, antibiotics, and the number of subculture generations influence somaclonal variations in torenia. The somaclonal variations in torenia may results from epigenetic changes.

References

Jan 17, 1997·The Journal of Biological Chemistry·F SchmittJ P Jost
Jun 29, 2004·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Mingliang XuSchuyler S Korban
Nov 30, 2006·Journal of Applied Genetics·Marcin Filipecki, Stefan Malepszy
Jan 4, 2008·BMC Plant Biology·Karsten OelkersTancred Frickey
Apr 22, 2010·The New Phytologist·Carlos M Rodríguez LópezMichael J Wilkinson

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
PCR
electrophoresis
scanning electron microscopy

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

© 2022 Meta ULC. All rights reserved