PMID: 16638281Apr 28, 2006Paper

Tissue culture of bovine lens as an in vitro model for posterior capsule opacification and the effects of pranoprofen on the cell confluence

[Zhonghua yan ke za zhi] Chinese journal of ophthalmology
Qiu-ming LiGuang-ying Zheng

Abstract

To establish an in vitro model of posterior capsule opacification (PCO) by culturing the posterior capsule of bovine lens, to observe the proliferation and differentiation of lens epithelial cells and to study the influence of serum and pranoprofen eyedrops on cell confluence of this model. The bovine lens posterior capsule was spread on the surface of a 25 ml culture flask with cell layer upward. DMEM with 0%, 10% and 20% fetal calf serum was used as culture medium. The cell coverage and confluence time on the posterior capsule were observed by inverted microscope and the cell morphology was observed by Giemsa staining and scanning electron-microscope. Pranoprofen was added to the culture medium at a concentration similar to the aqueous humor concentration (0.23 mg/L), which was presented at 4 hours after the instillation of pranoprofen eyedrops. The difference of confluence time between the treated group and the control group was compared. The lens epithelial cells migrated and proliferated rapidly on the posterior capsule from the equatorial region to the center. The cell coverage was increased and the confluence time was shortened with the increase of serum concentration (P < 0.05). The PCO and wrinkles were presented. Pran...Continue Reading

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Archives of Ophthalmology
N LoisJohn V Forrester
Investigative Ophthalmology & Visual Science
N LoisJohn V Forrester
Journal of Cataract and Refractive Surgery
M G DavidsonM C McGahan
Investigative Ophthalmology & Visual Science
Lucy J DawesI Michael Wormstone
© 2022 Meta ULC. All rights reserved