Tissue degeneration in ALS affected spinal cord evaluated by Raman spectroscopy

Scientific Reports
Gennaro PicardiAntonio Cricenti

Abstract

The Raman spectral features from spinal cord tissue sections of transgenic, ALS model mice and non-transgenic mice were compared using 457 nm excitation line, profiting from the favourable signal intensity obtained in the molecular fingerprint region at this wavelength. Transverse sections from four SOD1G93A mice at 75 days and from two at 90 days after birth were analysed and compared with sections of similarly aged control mice. The spectra acquired within the grey matter of tissue sections from the diseased mice is markedly different from the grey matter signature of healthy mice. In particular, we observe an intensity increase in the spectral windows 450-650 cm-1 and 1050-1200 cm-1, accompanied by an intensity decrease in the lipid contributions at ~1660 cm-1, ~1440 cm-1 and ~1300 cm-1. Axons demyelination, loss of lipid structural order and the proliferation and aggregation of branched proteoglycans are related to the observed spectral modifications. Furthermore, the grey and white matter components of the spinal cord sections could also be spectrally distinguished, based on the relative intensity of characteristic lipid and protein bands. Raman spectra acquired from the white matter regions of the SOD1G93A mice closely re...Continue Reading

References

Apr 12, 2005·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Christoph KrafftReiner Salzer
Sep 12, 2006·Trends in Molecular Medicine·Mariano S Viapiano, Russell T Matthews
Jun 19, 2008·Nature Clinical Practice. Neurology·Peter KühnleinAxel Riecker
Feb 13, 2009·Connective Tissue Research·Richard EllisC Peter Winlove
Feb 21, 2009·Nature Reviews. Neuroscience·Asya RollsMichal Schwartz
Mar 3, 2011·Journal of Biomedical Optics·Tarun SaxenaJoseph Chaiken
Oct 13, 2011·Nature Reviews. Neurology·Orla HardimanMatthew C Kiernan
Sep 14, 2012·Analytical Chemistry·Roberta GalliMatthias Kirsch
Sep 10, 2013·Neuroscience·S SolemanJ W Fawcett
Oct 15, 2014·Nature Reviews. Neurology·Bart Swinnen, Wim Robberecht
Mar 27, 2015·Advanced Drug Delivery Reviews·Kenny KongIoan Notingher
Dec 15, 2015·Analytical Chemistry·Thomas Wilhelm BocklitzJuergen Popp
Jun 30, 2016·Expert Opinion on Pharmacotherapy·H BlascoP H Gordon
Sep 3, 2016·Journal of Photochemistry and Photobiology. B, Biology·Shuang WangJintao Bai
Sep 18, 2016·Handbook of Clinical Neurology·E O TalbottD Lacomis
May 20, 2017·Lancet Neurology·UNKNOWN Writing Group, UNKNOWN Edaravone (MCI-186) ALS 19 Study Group
May 30, 2017·Lancet·Michael A van EsLeonard H van den Berg
Oct 6, 2017·Biophysical Journal·Paulina GaseckaSophie Brasselet

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic

Related Concepts

Related Feeds

ALS: Pathogenic Mechanisms

Amyotrophic Lateral Sclerosis is a progressive neurodegenerative disorder characterized by muscle weakness. Here is the latest research investigating pathogenic mechanisms that underlie this genetically heterogeneous disorder.

Amyloid Lateral Sclerosis

Amyotrophic Lateral Sclerosis (ALS) is a progressive nervous system disease associated with the death of neurons that control voluntary muscles. Discover the latest research on ALS here.