Tissue distribution and subcellular localization of the cardiac sodium channel during mouse heart development

Cardiovascular Research
Jorge N DomínguezAmelia E Aránega

Abstract

The aim of this study was to analyse the mRNA expression levels and protein distribution of the cardiac sodium channel Scn5a/Nav1.5 during mouse cardiogenesis. Scn5a mRNA levels were determined by real-time RT-PCR using embryonic hearts ranging from E9.5 to E17.5 as well as postnatal and adult hearts. In addition, Scn5a protein (Nav1.5) distribution was analysed by immunohistochemistry and confocal microscopy. Scn5a mRNA levels displayed a peak at stage E11.5, decreased during the subsequent stages and then steadily increased from E17.5 onwards, and throughout the postnatal to the adult stages. Immunohistochemistry experiments revealed comparable distribution of Nav1.5 between the different cardiac chambers at early embryonic stages. During the foetal stages, Nav1.5 showed an enhanced expression in the trabeculated myocardium and in the bundle branches. At the subcellular level, Nav1.5 and Scn1b double-immunostaining analysis is consistent with the presence of both sodium channel subunits in the T-tubule system and the intercalated discs. Our results demonstrate that the cardiac sodium channel, Nav1.5, shows a dynamic expression pattern during mouse heart development, indicating that it could play an important role in the acqui...Continue Reading

References

Oct 27, 1995·The Journal of Biological Chemistry·Y QuW A Catterall
Dec 1, 1995·Developmental Dynamics : an Official Publication of the American Association of Anatomists·B DelormeM Théveniau-Ruissy
Jan 1, 1996·Circulation Research·M P DaviesR S Kass
Jan 1, 1995·Reproduction, Fertility, and Development·J J Smolich
Jun 20, 1998·The Journal of Physiology·E MarbanG F Tomaselli
Sep 2, 1999·Nature Genetics·J J SchottH Le Marec
Jan 6, 2001·Neuron·A L GoldinW A Catterall
Mar 14, 2002·Proceedings of the National Academy of Sciences of the United States of America·Sebastian K G MaierWilliam A Catterall
Apr 25, 2002·Proceedings of the National Academy of Sciences of the United States of America·G Alex PapadatosAndrew A Grace
Oct 24, 2002·Journal of Molecular and Cellular Cardiology·Gui-Rong LiAlvin Shrier
Dec 25, 2002·Neuroreport·Ling WuQing Wang
Mar 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Sebastian K G MaierTodd Scheuer
Jun 14, 2003·Circulation Research·Fabien Brette, Clive Orchard
Dec 31, 2003·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·A Wessels, J M Pérez-Pomares
Oct 7, 2004·Circulation·William P McNairUNKNOWN Familial Cardiomyopathy Registry Research Group
Feb 22, 2005·Cardiovascular Research·Jorge N DomínguezAmelia E Aránega
Apr 29, 2006·Journal of Cardiovascular Electrophysiology·Cordula M Wolf, Charles I Berul
May 5, 2007·The Journal of Physiology·Nathalie GaboritSophie Demolombe
May 22, 2007·Cardiovascular Research·Michael HesseJames C Cross
Nov 1, 1994·Trends in Cardiovascular Medicine·A F Moorman, W H Lamers

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Citations

Mar 24, 2011·Cardiovascular Research·Diego FrancoAmelia Aránega
Sep 23, 2011·Cardiovascular Research·Martin B RookMarti F A Bierhuizen
Nov 24, 2012·Cardiovascular Research·Diego Franco
Aug 12, 2008·Circulation Research·Laura E BriggsHideko Kasahara
Jun 19, 2012·The Journal of Clinical Investigation·Malou van den BoogaardVincent M Christoffels
Jun 26, 2012·The Journal of Clinical Investigation·David E ArnoldsIvan P Moskowitz
Mar 13, 2012·Heart Rhythm : the Official Journal of the Heart Rhythm Society·Halina S ChkourkoMario Delmar
May 12, 2009·Journal of Molecular and Cellular Cardiology·Susann G KaufmannSebastian K G Maier
Oct 26, 2010·Birth Defects Research. Part B, Developmental and Reproductive Toxicology·Dominique AbelaWilliam S Webster
Aug 1, 2012·Progress in Biophysics and Molecular Biology·Catherine E MorrisBéla Joós
Feb 24, 2015·Heart Rhythm : the Official Journal of the Heart Rhythm Society·Houria DaimiDiego Franco
Aug 29, 2013·Journal of Molecular and Cellular Cardiology·Ruth E WestenbroekTodd Scheuer
Feb 3, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xaver Koenig, Karlheinz Hilber
Nov 25, 2016·Physiological Reviews·TingTing Hong, Robin M Shaw
Dec 1, 2018·American Journal of Physiology. Heart and Circulatory Physiology·Zhenwei PanBai-Yan Li
Aug 12, 2020·Europace : European Pacing, Arrhythmias, and Cardiac Electrophysiology : Journal of the Working Groups on Cardiac Pacing, Arrhythmias, and Cardiac Cellular Electrophysiology of the European Society of Cardiology·Mathilde R RivaudCarol Ann Remme
Jun 20, 2020·Journal of Internal Medicine·A van der PolP van der Meer
Jul 4, 2019·Biochimica Et Biophysica Acta. Molecular Cell Research·S H VermijJ P Kucera

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