PMID: 2506932Sep 4, 1989Paper

Tissue distribution of beta 1- and beta 2-subunits of regulatory guanine nucleotide-binding proteins

Biochimica Et Biophysica Acta
K D HinschG Schultz

Abstract

The beta-subunit of G-proteins occurs in two forms (beta 1 and beta 2), which differ in their primary structure as derived from cDNA clones and in their mobilities on SDS gels (36 and 35 kDa, respectively). To assess the tissue distribution of the two forms of beta-subunits, we synthesized peptides corresponding to defined regions of beta 1- and beta 2-subunits and injected them into rabbits; the antisera obtained reacted either with both beta-subunits or specifically with the beta 1- or the beta 2-subunit. They were used to identify the two beta-subunits in membranes prepared from various rat tissues and from human placenta. The concentration of total beta-subunits was high in rat brain and lung, human placenta, rat kidney, liver and spleen; it was much lower in rat erythrocytes, cardiac and skeletal muscle. In all tissues studied, both beta 1- and beta 2-subunits were detectable. In most tested tissues, the two forms were about equally distributed, whereas in the placenta, the beta 2-subunit was found to occur in approx. 2-fold excess over the beta 1-subunit. Our results demonstrate that both beta-subunits are widely distributed. In the majority of tissues, levels of beta 2-subunits are very similar to those of beta 1-subunit...Continue Reading

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·D E LogothetisD E Clapham
Jan 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·S M MumbyA G Gilman
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·P GierschikA Spiegel
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·C L Jelsema, J Axelrod
Jun 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·H K FongM I Simon
Sep 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·B GaoJ D Robishaw

❮ Previous
Next ❯

Citations

Aug 1, 1996·Journal of Photochemistry and Photobiology. B, Biology·W von der HeideH Stieve
Jan 18, 1994·Proceedings of the National Academy of Sciences of the United States of America·S OffermannsG Schultz
Apr 16, 1996·Proceedings of the National Academy of Sciences of the United States of America·U RudolphL Birnbaumer
Mar 1, 1995·Molecular Reproduction and Development·K D HinschE Hinsch
Sep 3, 1998·The Journal of Biological Chemistry·K Wenzel-SeifertR Seifert
Oct 7, 1998·Neurochemistry International·G FábiánM Szucs
Jan 1, 1994·Life Sciences·C W EmalaM A Levine
Mar 15, 1991·Biochemical and Biophysical Research Communications·K SpicherW Rosenthal
Sep 18, 2002·European Journal of Cell Biology·Ingrid PahnerGudrun Ahnert-Hilger
Jun 29, 1999·European Journal of Cell Biology·I BrunkG Ahnert-Hilger

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.