PMID: 6989265Mar 1, 1980Paper

Tissue distribution of glucagon, glucagonlike immunoreactivity, and insulin in the rat

The American Journal of Physiology
A Pérez-Castillo, E Blazquez


To show that glucagon, glucagonlike immunoreactivity (GLI), and insulin are synthetized by organs other than the pancreas and the gastrointestinal tract, different rat tissue acid-ethanol extracts were obtained and analyzed by immunoassay using specific antisera. Significant amounts of glucagon were found in the gastrointestinal tract (44.77 +/- 5.4 ng), salivary glands (1.50 +/- 0.17 ng), thymus (2.80 +/- 0.46 ng), thyroid (0.25 +/- 0.02 ng), and adrenal glands (0.25 +/- 0.06 ng). Whereas GLI appeared in the gut mucosa, adrenal and salivary glands, genuine insulin was detected only in the pancreas. Aliquots of the tissue extracts, fractionated on Bio Gel P 30 columns, gave a 3,500 mol wt immunoreactive (30 K) peak that behaved as pancreatic glucagon on acrylamide gel electrophoresis and displaced 125I-labeled glucagon previously bound to its hepatic receptors. Arginine, epinephrine, and low glucose concentrations stimulated glucagon release from parotid, thymus, and thyroid. Active glucagon biosynthesis by these organs was established by the incorporation of L-[3H]tryptophan into a 3,500 mol wt polypeptide with specific immune reaction with 30 K antiserum. These results suggest that different rat tissues can contribute to the ...Continue Reading


Feb 21, 1977·Biochemical and Biophysical Research Communications·S J BhathenaL Recant
Jul 1, 1975·The Journal of Clinical Investigation·H SasakiR H Unger
Jan 1, 1977·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·L Munõz BarraganR H Unger
May 1, 1976·The Journal of Cell Biology·D BaetensL Orci
Nov 1, 1976·Endocrinology·E BlazquezR H Unger
May 1, 1969·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·A G Pearse
Oct 1, 1965·The Journal of Clinical Endocrinology and Metabolism·V HerbertS J Bleicher
Sep 1, 1955·The Journal of Clinical Endocrinology and Metabolism·A J KENNY
Dec 28, 1964·Annals of the New York Academy of Sciences·B J DAVIS
Jun 1, 1965·Diabetes·B PANSKYL A CONE

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