Tissue distribution of human aldehyde dehydrogenase E3 (ALDH9): comparison of enzyme activity with E3 protein and mRNA distribution

Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology
G IzaguirreR Pietruszko

Abstract

The tissue distribution of the E3 isozyme of human aldehyde dehydrogenase has been investigated by three methods: enzyme activity assay employing betaine aldehyde as substrate, Western blotting employing E3 isozyme-specific antibodies, and Northern blotting using a human liver E3 cDNA as probe. All three methods showed that E3 isozyme was universally distributed among all tissues tested. The highest levels of the E3 isozyme activity were found in liver, adrenal gland, and kidney. These same tissues also showed highest levels of the E3 protein via the Western blot. This distribution is consistent with the possible physiological role of E3 isozyme in the synthesis of the osmolyte, betaine, and the neurotransmitter, GABA. Northern blot analysis, however, differed from that of enzyme assay and the Western blot in that it showed highest mRNA levels in skeletal and heart muscles, which had low enzyme activities and E3 protein levels.

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Citations

Oct 27, 1998·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·G IzaguirreR Pietruszko
Jan 23, 2002·The Biochemical Journal·Frédéric M Vaz, Ronald J A Wanders
Apr 1, 2015·PloS One·Marc Y R HenrionRichard S Houlston
Mar 5, 2003·Journal of Biochemical and Molecular Toxicology·Norman E Sládek
Nov 14, 2007·Toxicological Sciences : an Official Journal of the Society of Toxicology·Yazen Alnouti, Curtis D Klaassen
Jun 21, 2017·Biochemical and Biophysical Research Communications·Jesús Alfredo Rosas-RodríguezGuadalupe González-Ochoa
Mar 9, 2010·The Journal of Biological Chemistry·Chad BrockerVasilis Vasiliou
Jun 1, 2006·Molecular Reproduction and Development·Michael J MisamoreJohn W Lynn
Nov 29, 2017·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·S Ikeyama, Y Amao
Jan 4, 2021·Molecular and Cellular Biochemistry·Ramses Cruz-ValenciaElisa M Valenzuela-Soto
Jan 13, 2001·Chemico-biological Interactions·V VasiliouD R Petersen

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