PMID: 8972720Nov 8, 1996Paper

Tissue distribution of human gliostatin/platelet-derived endothelial cell growth factor (PD-ECGF) and its drug-induced expression

Biochimica Et Biophysica Acta
K MatsukawaT Kato

Abstract

Human tissue contents of gliostatin/platelet-derived endothelial cell growth factor (PD-ECGF) and its drug-induced expression in tumor cells were currently examined by a sandwich enzyme immunoassay (EIA) system and a reverse transcription-polymerase chain reaction (RT-PCR) method. Gliostatin/PD-ECGF was found to distribute in rather ubiquitous than specific human tissues and organs, with a relatively high levels in the tissues of digestive system (esophagus and rectum), brain, spleen, bladder and lung, but not in gall bladder, aorta, muscle, fat and kidney. Most of examined human tumor cell lines showed 4- or 5-fold higher contents (21.5 +/- 3.9 ng/mg protein) than normal tissue contents (4.4 +/- 1.1 ng/mg protein) on the average. While gliostatin/PD-ECGF is known to lack a signal sequence, some tumor cells (A431 and MKN74) appeared to release it into the conditioned medium. Expression of gliostatin/PD-ECGF in epidermoid carcinoma cell (A431) and stomach cancer cell (MKN45) was induced by dibutyryl cyclic AMP and phorbol ester, and uniquely in MKN45 by hydrocortisone. In particular, this hydrocortisone specifically caused an increase of the apparent secretion of MKN74 without its cytotoxic effects, suggesting a possible secreti...Continue Reading

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Citations

Jul 5, 2008·Nucleosides, Nucleotides & Nucleic Acids·I V BijnsdorpG J Peters
Sep 22, 2011·Brain : a Journal of Neurology·Caterina GaroneMichio Hirano
Aug 28, 2003·Clinical Chemistry and Laboratory Medicine : CCLM·Ramon MartíMichio Hirano
Jun 22, 2010·Mutation Research·Chiara RampazzoVera Bianchi
Jun 25, 2005·Gene·Michio HiranoYutaka Nishigaki
May 13, 2009·Medicinal Research Reviews·Annelies BronckaersSandra Liekens
Jun 19, 2007·Biochemical Pharmacology·Sandra LiekensJan Balzarini
Jul 16, 2003·Hypertension·Richard N Re
Sep 12, 1998·British Journal of Haematology·M P Bodger, D N Hart
Jul 25, 2000·Journal of the Peripheral Nervous System : JPNS·M J Eaton
Feb 13, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Richard N Re

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