Tissue distribution of the Ehrlichia muris-like agent in a tick vector

PloS One
Geoffrey E LynnU G Munderloh

Abstract

Human pathogens transmitted by ticks undergo complex life cycles alternating between the arthropod vector and a mammalian host. While the latter has been investigated to a greater extent, examination of the biological interactions between microbes and the ticks that carry them presents an equally important opportunity for disruption of the disease cycle. In this study, we used in situ hybridization to demonstrate infection by the Ehrlichia muris-like organism, a newly recognized human pathogen, of Ixodes scapularis ticks, a primary vector for several important human disease agents. This allowed us to assess whole sectioned ticks for the patterns of tissue invasion, and demonstrate generalized dissemination of ehrlichiae in a variety of cell types and organs within ticks infected naturally via blood feeding. Electron microscopy was used to confirm these results. Here we describe a strong ehrlichial affinity for epithelial cells, neuronal cells of the synganglion, salivary glands, and male accessory glands.

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Citations

Jan 30, 2016·Vector Borne and Zoonotic Diseases·Sandor E KarpathyMichael L Levin
Jan 2, 2016·Journal of Medical Entomology·Jonathan D OliverUlrike G Munderloh
Apr 22, 2015·Experimental & Applied Acarology·Jonathan D OliverUlrike G Munderloh
Jan 29, 2017·Parasites & Vectors·Geoffrey E LynnUlrike G Munderloh
Nov 15, 2017·Parasites & Vectors·Nídia CangiNathalie Vachiéry
May 12, 2019·Applied and Environmental Microbiology·Geoffrey E LynnUlrike G Munderloh
Oct 1, 2020·Scientific Reports·Jaroslav OndrušPavel Široký
Apr 23, 2021·Journal of Medical Entomology·Jean I TsaoGraham J Hickling

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Methods Mentioned

BETA
electron microscopy
PCR
electrophoresis
Assay
Illumina sequencing

Software Mentioned

SPAdes
Adobe Photoshop
ImageJ
Photoshop
MetaMorph Premier

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