Tissue distribution of the four gene products of the plasma membrane Ca2+ pump. A study using specific antibodies.

The Journal of Biological Chemistry
T StaufferErnesto Carafoli

Abstract

Antibodies against the four isoforms of the human plasma membrane Ca(2+)-ATPase (PMCA) were raised using an N-terminal sequence of the pump as epitope. The antibodies against PMCA isoforms 1, 2, and 3 were not species-specific, e.g. they also recognized the corresponding proteins in rat, whereas that against the human PMCA isoform 4 failed to do so. The tissue distribution of the four isoforms was estimated by Western blot analysis. Two, PMCA1 and PMCA4, were expressed in all tissues tested (with the exception of the choroid plexus, where the former was not detected). In most tissues the signal from the PMCA1 protein exceeded that of PMCA4, the exception being the erythrocyte. The PMCA2 and PMCA3 proteins were only found in neuronal tissues; the PMCA2 protein was present in high concentrations in the cerebellum and in the cerebral cortex. At variance with previous results on mRNA (e.g. the kidney) no other tissues contained the PMCA2 protein. PMCA3 was the other tissue-specific isoform; in agreement with results in the rat, the protein was found in human neuronal tissues, particularly in the choroid plexus, but was practically absent in all other tissues tested.

References

Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Dec 11, 1992·Brain Research. Molecular Brain Research·W L StahlW R Anderson
Apr 1, 1990·European Journal of Immunology·D StüberH Matile
Feb 1, 1991·The Journal of Membrane Biology·E E Strehler
Aug 1, 1989·The American Journal of Physiology·J L BorkeR Kumar
Jun 15, 1966·Experientia·H J Schatzmann
Feb 1, 1995·Brain Research. Molecular Brain Research·D A ZachariasE E Strehler
Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·N Tolosa de TalamoniJ T Penniston
Sep 30, 1993·Nature·M J Berridge
Jan 15, 1993·Brain Research·D BleakmanN L Harrison
Sep 1, 1993·Trends in Cardiovascular Medicine·E Carafoli, D Guerini

❮ Previous
Next ❯

Citations

Sep 5, 2002·The Journal of Comparative Neurology·David KrizajDavid R Copenhagen
Jun 9, 2001·Journal of Neuroscience Research·M L GarciaA J Windebank
Dec 22, 1999·Biochemical and Biophysical Research Communications·E CarafoliD Guerini
Oct 3, 2007·Pflügers Archiv : European journal of physiology·Magdalena M SzewczykAshok K Grover
Jun 13, 2008·Pflügers Archiv : European journal of physiology·Marisa Brini
May 1, 2005·Journal of Biological Physics·Michael GraupnerMichael Meyer-Hermann
Jul 26, 2011·Science China. Life Sciences·Elizabeth J CartwrightLudwig Neyses
Aug 14, 2003·Biochemical and Biophysical Research Communications·C MourreM Hugues
Jan 8, 1999·Neurobiology of Aging·A ZaidiM L Michaelis
Jul 17, 1998·Biochimica Et Biophysica Acta·D E Evans, L E Williams
Aug 6, 1999·Biochimica Et Biophysica Acta·D A Zacharias, C Kappen
Jun 22, 1999·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·M Alves-FerreiraA Ferreira-Pereira
Oct 15, 2003·Neuron·George J AugustineKeiko Tanaka
Oct 3, 2002·Cellular Signalling·Won Jae LeeGregory R Monteith
Jun 12, 2001·Current Opinion in Neurobiology·B L SabatiniK Svoboda
Aug 28, 1999·Journal of Pharmacological and Toxicological Methods·G R MonteithB D Roufogalis
Mar 5, 1999·Biochimica Et Biophysica Acta·L ZylińskaL Lachowicz
Aug 5, 2005·Visual Neuroscience·René C RenteríaDavid Krizaj
Aug 22, 2000·European Journal of Biochemistry·G E Shull
Feb 15, 2001·Liver Transplantation : Official Publication of the American Association for the Study of Liver Diseases and the International Liver Transplantation Society·P K JanickiC W Pinson
Jul 23, 2005·The Cerebellum·M Rosario Sepúlveda, Ana M Mata
Feb 1, 1997·The Journal of General Physiology·K E WhiteP A Friedman
Oct 14, 2006·Current Opinion in Urology·R P HolmesH O Goodman
Feb 27, 2009·Journal of Neurophysiology·Michael J FerragamoStanley A Thayer
Mar 30, 2001·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·P GlendenningR L Prince
May 4, 2011·World Journal of Biological Chemistry·Jyoti PandeAshok K Grover
Nov 4, 2000·Biochemical and Biophysical Research Communications·P GlendenningE E Strehler
Nov 26, 1996·Proceedings of the National Academy of Sciences of the United States of America·P C BrandtT C Vanaman
Mar 3, 2011·Neurochemical Research·Joachim KrebsMarek Michalak
Oct 9, 2012·The International Journal of Biochemistry & Cell Biology·Marta GiacomelloErnesto Carafoli
Oct 27, 2015·Frontiers in Synaptic Neuroscience·Thomas M BartolMary B Kennedy
Feb 14, 1997·Brain Research·T P StaufferE Carafoli
Apr 4, 2015·Organogenesis·Blanca Delgado-Coello, Jaime Mas-Oliva

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.