Tissue Engineered Esophageal Patch by Mesenchymal Stromal Cells: Optimization of Electrospun Patch Engineering

International Journal of Molecular Sciences
Silvia PisaniBice Conti

Abstract

Aim of work was to locate a simple, reproducible protocol for uniform seeding and optimal cellularization of biodegradable patch minimizing the risk of structural damages of patch and its contamination in long-term culture. Two seeding procedures are exploited, namely static seeding procedures on biodegradable and biocompatible patches incubated as free floating (floating conditions) or supported by CellCrownTM insert (fixed conditions) and engineered by porcine bone marrow MSCs (p-MSCs). Scaffold prototypes having specific structural features with regard to pore size, pore orientation, porosity, and pore distribution were produced using two different techniques, such as temperature-induced precipitation method and electrospinning technology. The investigation on different prototypes allowed achieving several implementations in terms of cell distribution uniformity, seeding efficiency, and cellularization timing. The cell seeding protocol in stating conditions demonstrated to be the most suitable method, as these conditions successfully improved the cellularization of polymeric patches. Furthermore, the investigation provided interesting information on patches' stability in physiological simulating experimental conditions. Cons...Continue Reading

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Citations

Nov 1, 2020·Biomaterials·Wissam FarhatAlexandra Fuchs
Apr 1, 2021·Microscopy and Microanalysis : the Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada·Constantine TarabanisElliot L Chaikof
Aug 11, 2021·Pharmaceutics·Sonia TrombinoFrancesca Iemma

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Methods Mentioned

BETA
biopsy
flow-cytometry
Precipitation
density gradient centrifugation
flow cytometry
Scanning
scanning electron microscopy

Software Mentioned

Graph Pad Prism
FAMAS
Cellcrow

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