Tissue-engineered mitral valve: morphology and biomechanics †

Interactive Cardiovascular and Thoracic Surgery
Pavel IablonskiiAxel Haverich

Abstract

The present study aimed at developing tissue-engineered mitral valves based on cell-free ovine mitral allografts. The ovine mitral valves (OMVs) (n = 46) were harvested in the local slaughter house. They were decellularized using detergent solutions and DNase. The effectiveness of decellularization was assessed by histological (haematoxylin-eosin, Movat's pentachrome) and immunofluorescent staining (for DNA and α-Gal), and DNA-quantification. To reveal the receptiveness of decellularized tissue to endothelial cells (ECs), the valve leaflets were reseeded with ovine ECs, derived from endothelial progenitor cells in vitro. For assessment of biomechanical properties, uniaxial tensile tests were carried out. Histology and immunofluorescent staining revealed absence of cell nuclei in decellularized leaflets, chordae and papillary muscles. According to the software for immunofluorescence analysis, reduction in DNA and α-Gal was 99.9 and 99.6%, respectively. DNA-quantification showed 71.2% reduction in DNA content without DNase and 96.4% reduction after DNase treatment. Decellularized leaflets were comparable with native in ultimate tensile strain (native, 0.34 ± 0.09 mm/mm, vs decellularized, 0.44 ± 0.1 mm/mm; P = 0.09), and elastin ...Continue Reading

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Citations

Sep 10, 2016·Tissue Engineering. Part a·Christopher DebordeAgneta Simionescu
Jan 13, 2018·Tissue Engineering. Part B, Reviews·Kevin M BlumChristopher K Breuer
Feb 1, 2018·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Pavel IablonskiiIgor Tudorache
Dec 21, 2018·International Journal of Molecular Sciences·Andrea PorzionatoRaffaele De Caro
Feb 27, 2017·Cell and Tissue Banking·M van SteenbergheP Gianello
Nov 18, 2018·The International Journal of Artificial Organs·Fabian KoenigNikolaus Thierfelder
May 4, 2017·Journal of Cardiovascular Translational Research·M GranadosSotirios Korossis

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