PMID: 15381713Sep 24, 2004Paper

Tissue-engineered rabbit cranial suture from autologous fibroblasts and BMP2

Journal of Dental Research
Liu Hong, J J Mao

Abstract

Craniosynostosis is a congenital disorder of premature ossification of cranial sutures, occurring in one of approximately every 2500 live human births. This work addressed a hypothesis that a cranial suture can be tissue-engineered from autologous cells. Dermal fibroblasts were isolated subcutaneously from growing rabbits, culture-expanded, and seeded in a gelatin scaffold. We fabricated a composite tissue construct by sandwiching the fibroblast-seeded gelatin scaffold between two collagen sponges loaded with recombinant human BMP2. Surgically created, full-thickness parietal defects were filled with the composite tissue construct in the same rabbits from which dermal fibroblasts had been obtained. After four-week in vivo implantation, there was de novo formation of tissue-engineered cranial suture, microscopically reminiscent of the adjacent natural cranial suture. The tissue-engineered cranial suture showed radiolucency on radiographic images, in contrast to radio-opacity of microscopically ossified calvarial defects filled with fibroblast-free, BMP2-loaded constructs. This approach may be refined for tissue engineering of cranial sutures for craniosynostosis patients.

References

Jan 1, 1982·Archives of Oral Biology·M C MeikleJ J Reynolds
Jun 20, 1995·Proceedings of the National Academy of Sciences of the United States of America·Y H LiuR E Maxson
Mar 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·C De PollackP J Marie
Mar 13, 1998·Anatomy and Embryology·B ZimmermanJ Bier
Oct 16, 1999·Journal of Morphology·K L Rafferty, S W Herring
Jun 14, 2000·Plastic and Reconstructive Surgery·J P BradleyM T Longaker
Sep 15, 2000·Plastic and Reconstructive Surgery·J A GreenwaldM T Longaker
Nov 21, 2000·Developmental Dynamics : an Official Publication of the American Association of Anatomists·L A Opperman
Jun 2, 2001·The Cleft Palate-craniofacial Journal : Official Publication of the American Cleft Palate-Craniofacial Association·M P MooneyM I Siegel
Jun 5, 2001·Nature Reviews. Genetics·A O Wilkie, G M Morriss-Kay
Dec 26, 2001·Yonsei Medical Journal·S M Warren, M T Longaker
Jun 28, 2002·Orthodontics & Craniofacial Research·D A PutzM P Mooney
Nov 28, 2002·Journal of Dental Research·J J Mao
Nov 29, 2002·Frontiers in Bioscience : a Journal and Virtual Library·Jeremy J MaoJames A Nudera
Mar 7, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Ross A Kopher, Jeremy J Mao
Apr 4, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Michael A IgnelziAndrew T Young
May 8, 2003·The Cleft Palate-craniofacial Journal : Official Publication of the American Cleft Palate-Craniofacial Association·Amr M MoursiMark P Mooney
May 22, 2004·Journal of Dental Research·P Radhakrishnan, J J Mao
Jun 5, 2004·American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, Its Constituent Societies, and the American Board of Orthodontics·Jeremy J Mao, Hyun-Duck Nah

❮ Previous
Next ❯

Citations

Oct 26, 2006·Journal of Dental Research·J J MaoS Shi
Dec 4, 2013·Tissue Engineering. Part a·Chang H LeeJeremy J Mao
Nov 6, 2013·Tissue Engineering. Part C, Methods·Chan Ho ParkWilliam V Giannobile
May 16, 2014·International Journal of Molecular Sciences·Rajesh L ThangapazhamJon Meyerle
Oct 14, 2011·Biomaterials·Chan Ho ParkWilliam V Giannobile
May 18, 2010·Biomaterials·Chan Ho ParkWilliam V Giannobile
Dec 8, 2009·American Journal of Orthodontics and Dentofacial Orthopedics : Official Publication of the American Association of Orthodontists, Its Constituent Societies, and the American Board of Orthodontics·Sean Shih-Yao LiuPeter H Buschang
Nov 21, 2007·Bone·Eduardo K MoioliJeremy J Mao
Feb 21, 2009·Journal of Oral Rehabilitation·E L SchellerD H Kohn
Jun 2, 2005·Biotechnology and Bioengineering·Mohamed N Rahaman, Jeremy J Mao

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
Robert E Guldberg
Nature Reviews. Molecular Cell Biology
Ryan J PetrieKenneth M Yamada
Proceedings of the National Academy of Sciences of the United States of America
Kristen L MoffatHelen H Lu
© 2021 Meta ULC. All rights reserved